多种致病菌同步检测技术在食品安全中的应用

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中图分类号：TS207.4 文献标志码：A 文章编号：1674-0270（2025）36-0111-03

Application of Simultaneous Detection Technology for Multiple Pathogenic Bacteria in Food Safety

GAOLi,YANGHaixia (Zaozhuang Vocational College, Zaozhuang 277Ooo, China)

Abstract: In response to the threat to public health and safety posed by multiple pathogenic bacteria in food and the limitations of traditional detection methods such as long detection cyclesand low eficiency,a technical system forsimultaneous detectionof multiple pathogenic bacteria is established toimprovedetection eficiency.Theresearch subjectsareSalmonella,Staphylococcus aureus,and Listeria monocytogenes.Specific primers and probesaredesigned, andasimultaneous real-timefluorescencePCRdetectionsystemisestablishedandoptimized.Methodologicalvalidation and practical application evaluation are conducted on samples such as meat products,dairy products,and aquatic products.The detection limit of the established method reaches 102 CFU/mL,with good specificity. The detection specificity for different target bacteria is 100% . The detection time is shortened to 4~6 hours.The validation of 12O actual samples showed that the coincidence rate with the traditional culture method is 96.7% . This simultaneous detection technology can overcome the shortcomings of traditional methods such as long time consumption and low throughput. It hastheadvantagesofrapidityaccuracy,andhighthroughput,providinganefective technical means forfoodsafety supervision and emergency monitoring.

Key Words: foodborne pathogenicbacteria; simultaneousdetection; real-time fluorescence PCR;rapid detection;foodsafety

等主要食源性致病菌，努力创建并完善多目标同步检测技术体系，旨在为食品安全监管提供高效且精确的技术支撑，满足其实际应用需求。（剩余4707字）