基层检测机构食品微生物指标快速检测方案构建

打开文本图片集

中图分类号：TS207 文献标志码：A 文章编号：1674-0270（2025）36-0092-04

Abstract： Toaddress isues suchas prolonged testing cycles，insuffcient equipment，weak technical adaptabilityand inadequate qualitycontrol systems in grasroots testing institutions，this paper proposes an innovative and practicalrapid testing solution.Centeredon "multi-technologycollboration-process optimization-three-dimensionalqualitycontrol，" the solution integrates technologieslikebiosensors，LAMPisothermalamplification，and microfluidicchips tostablish a multi-indicatorsynchronous detection system.Itoptimizes the entire workflowand forms aclosed-loopquality control mechanism.Validationresults showthat the solution reduces testing cycles forfood microbiological inspection items such as total bacterialcount，coliforms，moldsand yeasts，and commercial sterilityto2to5hours.Pathogenic bacteria detection limits reach 1O CFU/mL，while mycotoxin detection limits achieve 0.1μg/kg ，witha method specificityof 98 % . The coincidence rate with national standard methods is ⩾95% . The solution is simple to operate， cost-effective， and tailored to grassots needs，providing technical support and a practical paradigm for enhancing foodsafety at the grassroots level.

Key Words： grassroots testing institutions; food microbiology;rapiddetection; scheme construction;qualitycontrol system

食品微生物污染是食源性疾病的主要诱因，基层检测机构作为防控“第一道防线”，直接关系公众健康与监管实效。（剩余5108字）