天然油脂中留醇的分析方法及鉴别

打开文本图片集

关键词：气相色谱法；甾醇组成；化学计量学；天然油脂中图分类号：TQ645 文献标志码：A 文章编号：1671-0460（2026）01-0246-07

Abstract： Ananalysis methodfor assssing the compositionand massfraction ofsterols was developed，and natural oils andfats were differentiatedbyusingchemometric techniques. Unsaponifiable fractions were extractedfrom the oilsand fats，and sterol fractions were separated by liquid chromatography.These fractions were subsequently derivatized to facilitate the determinationofsterolcompositionand mass fraction bygaschromatography.The sterolcompositionand massfraction in 27 batches of9 diferent natural oils and fats were analyzed employing principal component analysis （PCA）and cluster heat map analysis.The results showed that the method had high specificity fordetermining sterol composition and mass fraction in oils andfats， withrelative standard deviations （RSD）forrepeatabilityand intermediate precision being less than 5% .The test solution remained stable for up to 36h ，and minor variations in chromatographic conditions had minimal impact onthedeterminations，enhancing the method's robustnes.The integrationof these two chemometric methods enabled visual analysis ofoil types，effectively identifying and distinguishing various natural oils and fats.

Keywords： Gas chromatography; Sterol composition; Chemometrics; Natural oils and fats

天然油脂中含有比较丰富的甾醇，按照来源可分为动物性甾醇、植物性甾醇和菌类甾醇三大类，其基本结构为环戊烷多氢菲，含有醇基，因此被称为甾醇类化合物。（剩余8116字）