高效液相色谱法测定糕点中合成着色剂的影响因素及优化策略研究

关键词：高效液相色谱法；合成着色剂；糕点；影响因素；优化策略

中图分类号：TS207.3 文献标志码：A 文章编号：1674-0270（2026）01-0125-04

DOl： 10.16043/j.cnki.cfs.2026.01.032

Research on Influencing Factors and Optimization Strategies for Determination of Synthetic Colorants in Pastries by High-Performance Liquid Chromatography

LIN Xiaofei'， WU Huanxin²， LIPing （1. Jiande Food and Drug Inspection and Testing Center， Jiande 3116Oo， China; 2.Hangzhou Jiande Ecological and Environmental Monitoring Station， Hangzhou 3116Oo， China）

Abstract： Synthetic colorants are widely used in pastry production， and their excessive application poses a significant threat to food safety，making it essential to establishaccurateand reliable detection methods.Highperformance liquid chromatography （HPLC） is employedas thecore analytical approach to systematically investigate the effcts of sample pretreatment methods，chromatographic condition selection，matrix complexityanddetection wavelength setings on detection outcomes， with corresponding optimization strategies proposed for each identified influencing factor. Given the complex nature ofthe pastry matrix，particular attention is paid to the critical steps affecting method accuracy and reproducibility. The results demonstrate thatthe optimized pretreatment procedure and chromatographic parameter configuration effectively eliminate matrix interference，and the introductionof a programmed wavelength-switching detection mode further improve the signal response sensitivity of each component， significantly enhancing the sensitivity and accuracy of the method.The optimized HPLC method exhibits good linearity and satisfactory recovery rates，providing reliable technical support for routine monitoring of synthetic colorants in pastries and food safety supervision.

Key Words： high-performance liquid chromatography; synthetic colorants; pastries; influencing factors; optimization strategies

合成着色剂因色泽鲜艳、稳定性强、成本低廉，广泛应用于糕点类食品的生产加工过程中，常见的合成着色剂品种有苋菜红、胭脂红、柠檬黄、日落黄等。（剩余5197字）