基于呼吸代谢特征的益生菌活性快速检测方法研究

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关键词：益生菌；活性检测；呼吸代谢；气相色谱；二氧化碳

中图分类号：TS201.3 文献标志码：A 文章编号：1674-0270（2026）01-0073-04

DOl： 10.16043/j.cnki.cfs.2026.01.019

Research on Rapid Detection Method of Probiotic Activity Based on Respiratory Metabolic Characteristics

ZHOU Chenhao （Wecare Probiotics （Suzhou） Co.，Ltd.， Suzhou 2152Oo， China）

Abstract： Probiotic activityisa keyindicator forevaluating its eficacy.Traditional plate counting methods onlyreflect the number of viable bacteria and cannot reflect the metabolic activityof the bacteria. Microorganisms produce CO 2 during metabolism，and the amount produced is directlyrelated to bacterial activity. Gas chromatography was used to detect the CO2 concentration in the probiotic metabolism， and a standard curve was constructed to achieve quantitative analysis of activity，with μg⋅CO2⋅g-1⋅d-1 as the unit of activity evaluation. The method was validated on Lactobacillus acidophilus LA85 powderand Pediococcus acidilactici PA53 granules.Theresults show thatthis method can effectively distinguish the activity differencesbetween diferent batches of products，withadetection limit of O.O05 ppm（（V/V） CO2 .Compared with traditional methods，this method has theadvantages of simple operation，low cost，and accurate results， providing a new technical means for probiotic quality control.

Key Words： probiotics; activity detection; respiratory metabolism; gas chromatography; carbon dioxide

益生菌能改善肠道微生态平衡，促进营养吸收并增强机体免疫力。（剩余5268字）