高效液相色谱法在食品中苯甲酸、山梨酸、安赛蜜及糖精钠检测中的应用价值

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关键词：高效液相色谱法；食品；苯甲酸；山梨酸；安赛蜜；糖精钠检测中图分类号：TS207.3 文献标志码：A 文章编号：1674-0270（2026）01-0065-04DOl: 10.16043/j.cnki.cfs.2026.01.017

The Application Value of High-Performance Liquid Chromatography in the Detection of Benzoic Acid, Sorbic Acid, Acesulfame Potassium and Saccharin Sodium in Food

WANG Yi (Chongzhou Center for Disease Control and Prevention, Chengdu 611230, China)

Abstract: To determine four food aditives,namely benzoicacid,sorbicacid,acesulfame potassiumand saccharin sodium i food by high-performance liquid chromatography (HPLC),thispaper establishes ananalytical procedure,and evaluates itsperformanceinrealfod matrics.IntheHPLCdetectionofbenzoicacid,sorbicacid,acesulfamepotssium and saccharin sodium in food, a C18 reversed-phase column was used,with a mobile phase system consisting of methanol and 2 mmol/L formic acid-20 mmol/Lammonium acetatebufer solution,and an isocratic elution program was implemented.Diferentpretreatmentsteps including degassing,protein precipitation,solid-phase extractionandsolvent extraction were caried out for liquid,semi-solid and solid samples.The method was validated for spiked recoveries and precision,and was applied to the detection of 6O real market samples.Results show thatthe average recoveries of benzoic acid, sorbic acid, acesulfame potassium and saccharin sodium at the addition levels of 5.0～100.0mg/kg are （2 88.2% .102.4% . In the real samples,at least one additive is detected in 39 samples,and the content of all additives in all samples does not exced the national standard limits.The HPLC method established inthis study is suitable forthe simultaneous detectionofbenzoic acid,sorbic acid,acesulfame potassum andsaccharinsodum invariouscomplexfood matrices,and has strong practicality,providing a basis for the supervision and evaluation of food additives.

Key Words: high-performance liquid chromatography; food; benzoic acid; sorbic acid; acesulfame potassium; saccharin sodium detection

食品安全是关乎公众健康和社会稳定的重要议题。（剩余6107字）