海南霸王岭不同菌根树种主导的真菌群落组成

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中图分类号：U455.6 文献标志码：A 文章编号：2096-9902（2026）06-0035-06

Abstract：Inmountainoushigh-altitudeareas，ectomycorhizal （ECM）treespecieshavesignificantadvantagesoverendophytic arbuscularmycorrhizal（AM）treespecies.However，thereisstillalackofsystematicresearchonthefactorsdrivingthis transformation.Inthisstudy，7ECMand6AMtreespecieswereselectedinBawangling，Hainan，andrhizospheresoilwas colectedintheirrespectivedisributionaltituderanges（owest，mostsuitable，andhighestboundaries），andanalyzedusingIS high-throughputsequencing.Theresultsshowedthathosttreespeciesidentitywasthemostimportantfactorinthedierentiation of rhizosphere fungal communities （ R2 =0.140），which was significantly higher than that of the altitude distribution range （ R2 =0.097） and mycorrhizal type（ R2 =0.015）.ECM tree species maintained a higherrelative abundance of rhizosphere fungi at the highest altuderangeoftheirdistribution，indicatingthattheycanefectivelywithstandenvironmentalpressurecausedbyrisingaltitude （GLMM interaction term Estimate=+0.012， P <0.001）. This reveals the ability of ECM tree species to maintain stable symbiotic relationshipsintherhizosphereatighaltitudesfromamicrobialperspective，highlightstheleadingroleoftreespeciesientity inthecomposionofrhizospherefungalcommunities，andprovidesasubterraneanecologicalbasisforexploringthedynamic changes in tropical mountainous forests.

Keywords：tropicalrainforest;rhizosphere fungal community；ectomycorhizal;arbuscular mycorhizal;altituderange

高通量测序技术的快速发展为根际真菌群落研究提供了强大工具，其中fastp可实现超快速的序列质控，FLASH用于双端读长的高效拼接，UPARSE能够从扩增子数据中生成高精度的OTU/ASV，经典的DNA-DNA杂交结合ITS序列分析仍是真菌分类学的基础，RDPClassifier基于朴素贝叶斯算法实现快速精确的物种注释，PICRUSt2则进一步预测群落潜在代谢功能。（剩余9293字）