UPLC一MS/MS法测定水产养殖水中 酰胺醇类药物残留的改进

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中图分类号：0657.6 文献标志码：A 文章编号：1004—6755（2026）04—0013—04

Improvement of UPLC-MS/MS method for determining mphenicol Residues in aquaculture water

CHENYongping1，XIA Sudong²，CHENG Zhenhua1，MA Chao¹， HAN Xianqin1，SHI Wenbo1，WANG Yuanning1，SU Menghan1 （1.TianjAgriculturalEcologicalEnviroentonitringandAgriculturalProductsQualitystingCenterianji3Oina TianjinAgriculturalCollege，Tianjin3oo384，China）

Abstract：A detection method for amphenicol antibiotic residues，chloramphenicol，methicilin，and florfenicol residues in aquaculture water based on rough active sampler-solid phase extraction- UPLC-MS/MS was improved. After filtration，the aquaculture water sample was filtered and cleaned up by Phenomenex SPE，eluted by 5mL methanol at a rate of 1.0mL/min ，dried by nitrogen gas and redissolved. The separation was performed using Waters ACQUTY UPLC HSS C18 （ 100mm×2.1 mm i. d.， 1.8μm ） in gradient elution with methanol-water as the mobile phase. The samples were detected by multiple reaction monitoring （MRM） ESI- mode and quantified by internal standard method. Chloramphenicol and methicillin achieved a good linearity in the range of 0.2～10ng/mL the linearity of florfenicol was in range of 0.1～5.0ng/mL ，with a correlation coefficient r2>0. 99 ： The detection limit of chloramphenicol and methicillin in actual spiked samples was 0.2ng/L ，the quantification limit was 0.5ng/L ，and the average recoveries range was between 81.2% and 94.7% ： The detection limit of florfenicol in actual spiked samples was 0.1ng/L and a quantification limit of 0.25ng/L ， with average recoveries range between 84.5% and 95.7% . This method has high accuracy and short detection time，and is suitable for simultaneous determination of chloramphenicol，methicillin，and florfenicol in aquaculture water.

Key words： aquaculture water; chloramphenicol; high- volume injection; solid- phase extraction; UPLC-MS/MS

氯霉素、甲矾霉素和氟苯尼考是酰胺醇类抗生素，因其对部分细菌病可产生抑制作用，养殖者常用于预防或治疗水产养殖中水生动物等疾病。（剩余4694字）