不同激素配比对吴起楸子试管苗离体生长及扩繁效率的影响研究

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中图分类号：S661.1 文献标识码：A

Effects of Different Hormone Ratios on In Vitro Growth and Propagation Efficiency of Malus prunifolia Plantlets

WANG Ying，MA Jianfang，YANG Junxia (Tianshui Research Institute of Pomology，Tianshui Gansu 74loo2，China)

Abstract:【Objective】To optimize the invitrorapid propagation system for Malus prunifolia fromWuqi,this study sys tematically investigated the regulatory effects of 6- Benzylaminopurine (6-BA) and auxin（IBA） concentration ratios in MS medium on the growth characteristics，propagationeficiency，and physiological indicators of tube plantlets.【Methods】A two-factorcompletelyrandomized design was employed，withfiveconcentrationsof6—BA（O.4，0.5，0.6，0.7，0.8mg/L) and five concentrations of IBA(0.5，0. 6,0.7,0.8, 0.9mg/L) ，forming lO treatment combinations ( T1-1 toT ). A hormone-free MS medium served as the control (CK).After 30 days of culture，parameters such as propagation coeficient, plant height，stem diameter，internode length，and chlorophyllcontent were measured. 【Results】The ratio of 6- BA to IBA significantly influenced the growth of tube plantlets. The combination of 0.7mg/L 6-BAand 0.6mg/L IBA (T5-2) ）yielded the highest propagation coefficient （14.125），while 0.5mg/L 6-BA and 0.9mg/L IBA (T5-1) resulted in the best performance in stem diameter and chlorophyllcontent.【Conclusion】Principal component analysis indicated that a 6- BA/IBA ratiobetween1.Oand1.2（e.g.，.17）synergisticallyenhanedproiferationeficiencyandphysilogicalindicators，fectively addressing the issue of excessive elongation (internode length

Key words:Malus prunifolia；in vitro plantlets；hormone optimization；propagation coeffcient；principal component analysis

吴起楸子（Malusprunifolia）作为我国西北 地区重要的苹果砧木资源，因其突出的抗寒性、抗

旱性及与栽培品种的良好嫁接亲和性，已成为黄土高原果园更新改造的核心砧木类型[1-2]。（剩余6519字）