核桃青皮提取物对苹果腐烂菌抑制作用研究

打开文本图片集

中图分类号： S436.611+11 文献标识码：A

Abstract：【Objective】Green husks of mature walnuts were extracted usingdiferent extractants，and theinhibitory effect of walnutgreen husk extracts onValsa mali （the pathogencausing apple valsa canker）was investigated，so as to provide theoreticalsupport forthedevelopmentofbacteriostatic agents basedon thereuseof waste walnut green husk resources.【Methods】Taking Valsa malias the testtarget，the grenhusks of mature walnuts were extracted with petroleum ether，ethylace tate，methanol and ethanol at different concentrations （ 25% ， 50% ， 75% ， 100%） . The obtained walnut green husk extracts were thenused todeterminetheirinhibitory efects on the mycelial growthofValsa mali.【Results】Theextracts obtainedusing methanolas theextractant showed thebest extraction eficiencyforthebacteriostaticcomponentsagainst Valsa maliThe bacteriostatic substances in walnut green husks included both water-solubleandorganic solvent-solublecompounds，withthe optimal extraction efectachieved when the volumes of waterand organic solvent were equal.【Conclusion】The walnut green husk extract obtained by 50% methanol exhibited the strongest inhibitory effect on Valsa mali，with the maximum inhibition rate reaching 88.7% and an EC5O value of 0.223.

Keywords：walnut；greenhuskextract;Valsa mali；inhibitionrate

苹果腐烂病是由苹果黑腐皮壳菌（ValsamaliMiyabeetYamada.）引起的一种病害，常在 5～ 6年生初结果的果树上发病。（剩余4613字）