地顶孢霉培养物中麦角留醇定量分析方法的建立与验证

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中图分类号 0657.72 文献标志码A 文章编号 1007-7731（2026）02-0092-05

DOI号 10.16377/j.cnki.issn1007-7731.2026.02.023

Development validation of quantitativemethod for ergosterol inAcremoniumterricolaculture

Xu Juanjuan1LiJunhui²Liu Faquan³Zhou Qun1Pan Hanwen1Wu Hao³Xu Ziao1 （1Hefei Co.，Ltd.， Hefei 2312Oo， China; ²Anhui Studying Technology Co.，Ltd.，Hefei 230031，China; 3Anhui Province Veterinary Drug Feed Supervision Institute，Hefei 23O091， China）

AbstractTo establish anaccurate reliable method for the quantitative analysis of ergosterol in Acremonium tericolaculture，thisstudyoptimizedthedetection wavelength，extractiontime（15，25，35，45，55min），extraction solvent （methanol，absolute ethanol， 95% ethanol），mobile phase ratio （methanol：water，100：0，98：2，95：5，90：10）， flow rate （0.6，0.8，1.0，1.2，1.4mL/min）basedon the conventional high-performance liquid chromatography （HPLC）， subsequentlyvalidatedtheoptimizedmethodintermsofspecificity，linearityprecision，accuracylimitofdetection （LOD） limitof quantification （LOQ），verifiedthe aplicabilityof the method using 10 batches of Acremonium tericola culture samples;theresultsshowed that the optimal conditions of the established HPLC method were as follows： detection wavelength at 281 nm， extraction solvent of 95% ethanol， extraction time of 45 min， mobile phase ratio of methanol to water at 100÷0 ， flow rate of 1.O mL/min，this method could effectively separate ergosterol from other components inAcremonium tericola culturewithout interfering with thedeterminationresultsof ergosterol，ergosterol exhibited a good linear relationship in the concentration range of 0.5-40.0μg/mL （ R2=0.999 2 ）， the relative stard deviation （RSD） of precision was less than 3% ，the recovery rate ranged from 90.45% to 99.41% ， the LOQ was

0.50μg/ml ， the LOD was 0.15μg/mL ，thedetection results of Acremonium terricola culture samples from the same batch showed goodrepeatability，indicating thatthemethod had high stabilityoperability; inconclusion，the HPLC method established in this studyhas the advantages of wide determination range，good linearity high accuracy， which can be used for the accurate quantification of ergosterol in Acremonium terricola culture.

KeywordsAcremonium terricola culture; ergosterol; HPLC; quantitative analysis; method validation

地顶孢霉培养物（Acremonium terricolaculture，ATC）是用从古尼虫草分离的地顶孢霉经液体一固体双相发酵而得到的产品，具有麦角甾醇、腺苷、虫草素、虫草酸、多糖、氨基酸等与古尼虫草相似的生物活性物质，其作为功能性饲料添加剂核心成分，可促进养殖动物生长、提升其机体免疫力。（剩余5697字）