基于芬芥素合成的解淀粉芽孢杆菌内源启动子筛选与功能验证

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关键词：芬芥素；解淀粉芽孢杆菌（Bacillusamyloliquefaciens）；内源启动子；筛选；功能验证

中图分类号：Q933 文献标识码：A

文章编号：0439-8114（2026）02-0209-06

DOI：10.14088/j.cnki.issn0439-8114.2026.02.031 开放科学（资源服务）标识码（OSID）：

Screeningand functional validation of endogenous promoters from Bacillus amyloliquefaciens forfengycin synthesis

ZHOULu，TIANYuan，YUChong，XIAHai-hua，YANGeng-xuan （Institute of Microbiology，Heilongjiang Academy of Sciences，Harbin 15oo1O，China）

Abstract：UsingBacilusamloliquefaciens28astehost，ndbyintegratinggenomicandtranscriptoicdata，2upstrado enouspromotersfromhighlyexpressedgeneswerescreeed.ByconstructingaGFPreportersystemandevaluating theactivityofeach promoterinBacillus，itwasfoundthathepromoterstrengthsofPspoVG，PqoxC，PqoxB，andPgpdwerehigherthanthatoftecommonlyusedpromoterP43.WhendrivenbythePspoVGpromoter，thetranscriptionallevelofthefenCgenewas4.62timesthaofthe P43 promoter，and the fengycin yield increased from 84.3mg/L to 101.2mg/L .Regarding promoter structure，the sequences of the -35 and -10 regions ofPspoVG were highlyconsistent with theconsensussequences oftypicalstrong promoters，which mightbethestructural basis for its high-eficiency transcriptional drive.

Key Words：fengycin;Bacillus amyloliquefaciens;endogenous promoter; screening; functional validation

芬芥素（Fengycin）也称丰原素，是一类由芽孢杆菌通过非核糖体肽合成酶途径合成的环状脂肽类化合物1。（剩余9920字）