芽孢杆菌合成纳米硒的物理化学表征及其应用于富硒番茄栽培

打开文本图片集

关键词：芽孢杆菌（Bacilluscereus）；纳米硒；物理化学表征；富硒番茄中图分类号：Q939.124 文献标识码：A文章编号：0439-8114（2026）02-0113-07DOI：10.14088/j.cnki.issn0439-8114.2026.02.017开放科学（资源服务）标识码（OSID）：

Physicochemicalcharacterization of nano-seleniumsynthesized by Bacillus and its application in selenium-enriched tomato cultivation

MADi，SHENG Qi，KANGuo-shi，CHENHong-man （Collge of Bioscience and Biotechnology，Shenyang Agricultural University，Shenyang 11O866，China）

Abstract：ASNPs-synthesizingBacteriumMC-3wasisolatedfromserenium-richsoilandidentifiedthroughmorphologicalobservationandl6SrDNAsequencinganalysis.ThephysicochemicalcharacteristicsofSeNPssynthesizedbystrainMC-3wereanalzedby scaningelectronmicrosope（SE），nergydispersivespecrometer（EDS），X-raydiracti（XRD），ndFouriertransfofrard spectroscopy（FTIR），and SeNPswereappliedtothecultivationexperimentofselenium-enrichedtomatoes.Theresultsshowedthat strainMC-3wasBacilluscereus.TheSeNPsfromreductionofN a2Se03 by B .cereusMC-3 presented both crystalline andamorphous selenium nanoparticles，withadiameterrangeofO0～30Onm，andtheirmoleculecontainelementssuchasC，O，andSe，aswell as functional groups such as-OH，C=O，and C-O，which were relatedto the stabilityof SeNPs.The SeNPs synthesized by B .cereus MC-3 wereusedas foliarfertilizerinthecultivationof selenium-enriched tomatoes.Whentheconcentrationof SeNPs was 70mg/L ， theselenium content in tomato fruits could reach 0.090mg/kg FW.Meanwhile，the contents of solubleprotein and vitaminCinthe fruits were 41.25% and 35.00% higher than those in the control group，respectively. In conclusion，the appropriate concentration of SeNPscouldbesprayedasfoliarfertilizerforselenium-enrichedtomatocultivation，andcouldimprovethequalityoftomatofruit.

Key Words： Bacillus cereus;nano-selenium;physicochemical characterization ;selenium-enriched tomato

硒是人体微量营养素，具有重要的生理功能[2]。（剩余11047字）