外源dsRNA介导的RNA干扰靶向沉默CP基因抑制烟草马铃薯Y病毒侵染

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关键词：烟草马铃薯Y病毒（PVY）；双链RNA（dsRNA）；RNA干扰（RNAi）；衣壳蛋白（CP）；生物防治

中图分类号：S435.72；Q78；S188 文献标识码：A

文章编号：0439-8114（2026）02-0101-06

DOI：10.14088/j.cnki.issn0439-8114.2026.02.015 开放科学（资源服务）标识码（OSID）：

Exogenous dsRNA-mediated RNA interference suppresses potato virus Y（PVY） infection intobaccobytargetingandsilencingthe CP gene

XURu-bing1，LUO Zhao-peng²，LI Yan-yan1，LIUJun-xia²，YANGJun²，QINYao1 （1.TobacoResechsiutefuberova0i;ecseahse，Zgo4 China）

Abstract：Toevaluatetheinhibitoryefectofexogenousdouble-strandedRNA（dsRNA）-mediatedRNAinterference（RNAi）onpotato virus Y（PVY） infection in tobacco，the coat protein（ CP ） gene of PVY was cloned.Subsequently，adsRNA fragment targeting this gene was designed based on sequence analysis.A 474-bp dsRNA targeting the PVY CP gene was synthesized in vitro and applied together with PVY onto tobacco plants.The relative mRNA level of the CP gene in the inoculated leaves was determined by quantitativereal-timePCR（qRT-PCR），whileviralsymptomdevelopment wasobservedovertime.Boththedisease incidenceanddisease indexwererecordedtocomprehensivelyevaluatethecontroleficacyofthedsRNAtreatmentagainstPVY.Theresultsshowedthatexogenous application of PVY CP gene targeting dsRNA significantly suppressed the expression of the PVY CP gene in the inoculated leaves and effectively alleviated virus-induced symptoms，resulting in a control efficacy of 50.58% in pot trials.

Key Words： potato virusY（PVY）；double-strandedRNA（dsRNA）；RNA interference（RNAi）；coatprotein（CP）； biocontrol

病毒病在烟草（NicotianatabacumL.）上普遍发生，其中，烟草马铃薯Y病毒（PotatovirusY，PVY）对烟草的安全生产构成了严重威胁，在中国多个烟区广泛分布，严重影响着中国烟草产业的发展[12]。（剩余9357字）