基于多酶恒温快速扩增检测猪链球菌及其3种主要血清型方法的建立及应用

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中图分类号：S852.611 文献标志码：A 文章编号：0366-6964（2026）01-0564-05

Abstract： Streptococcus suis （SS） is one of the important pathogens causing serious economic losses in the pig farming industry. Here，we developed a novel rapid detection method for detecting SS and serovars 2，7 and 9 based on multienzyme isothermal rapid amplification（MIRA） technology. The MIRA assays were validated for detection of SS and it serovars within 5 to 30min at 20-41∘C ，with relative higher specificity and sensitivity. There are 100% agreement between our MIRA and PCR methods in the detection of clinical samples. Our results showed that the MIRA was a useful diagnostic method for rapid diagnosis of SS infection.

Keywords：Streptococcus suis;serovars;MIRA;rapid detection ∗ Corresponding author： LIU Xuelan， E-mail： liuxuelan2O3@l63.com

猪链球菌（Streptococcussuis，SS）是影响全球养猪业的重要病原体之一，同时也是一种影响公共卫生的病原体。（剩余7999字）