15种血清型的鸭疫里默氏杆菌毒力基因检测和耐药性分析

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中图分类号：S852.61 文献标志码：A 文章编号：0366-6964（2026）01-0360-09

Abstract： In order to understand the drug resistance and virulence gene distribution of Riemerella anatipestifer isolates in some areas of China，K-B method was used to determine the sensitivity of 15 serotypes of R ： anatipestifer to 1O commonly used antibiotics，and PCR technology was used to detect 16 drug resistance genes and 16 virulence genes of different serotypes of R .anatipestifer isolates. The results showed that the resistance rates of amoxicillin and ampicillin were 93.33% and （204号 80% ， respectively. The detection results of drug-resistant genes showed that the detection rates of （204 tet（X） gene of tetracycline，floR gene of amide alcohol and aϕh（3′）-I gene of aminoglycoside were （204号 93.33% ， 80% and 73.33% respectively. The results of virulence gene detection showed that tctR ， TbdR1， TR ， CAMP and AS87-O4O5 had the highest detection rates，which were 86.67% ， （204号 86.67% ， 80% ， 73.33% and 73.33% respectively. These isolates are sensitive to tetracyclines， quinolones and amide alcohols，and have high resistance to β -lactams. At the same time，these isolates carry a variety of virulence genes，which may indicate that they have strong virulence. The above results provide some reference for the prevention and control of R . anatipestifer in some areas of China.

Keywords： Riemerella anatipestifer; virulence gene; drug resistance gene; drug-fast ∗ Corresponding author： YANG Min，E-mail：yummyl2@163.com

鸭疫里默氏杆菌（Riemerellaanatipestifer，RA）是引起鸭、鹅等水禽鸭疫里默氏菌病的病原菌，近年来也有引起鸡群发病的报道[1]。（剩余8884字）