过氧化物酶体增殖物激活受体α通过调控AMPK/P38MAPK通路促进小鼠骨骼肌发育

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关键词：不同发育阶段；过氧化物酶体增殖物激活受体 α（PPARα ；骨骼肌;AMPK;P38MAPK中图分类号：S852.2；R337 文献标志码：A 文章编号：0366-6964（2026）01-0443-11

Abstract：The aim of this study was to explore the effect and molecular mechanism of peroxisome proliferator-activated receptor α （ PPARα ）on skeletal muscle development. We utilized young（6-8 weeks），adult（3-4 months），and old（18-2O months） mice （ n=8/group ）to examine the expression of PPAR α in skeletal muscle during development. The role of PPARα in skeletal muscle development was further investigated using a whole-body knockout mouse model. HE staining was used to observe the morphological diferences of gastrocnemius muscle of mice of different ages，and immunofluorescence staining was used to detect the expression changes of P2l and P53 in gastrocnemius muscle of mice of different ages to determine the age difference of mice. The expression of PPARα in skeletal muscle was detected by common PCR，and the expression of PPARα in gastrocnemius muscle of mice of diferent ageswasdetectedby Westem blot， real-time quantitativePCR（qRT-PCR）and immunofluorescence staining （IF）. qRT-PCR，Western blot and immunofluorescence staining were used to detect the expression of MyoD，MyoG and Pax7 in gastrocnemius muscles of WT and PPAR α knockout mice （ PPARα-/- ）.Western blot was used to detect the expression of PPAR α downstream signaling pathways AMPK and P38 MAPK. The results showed that PPARα was expressed in the skeletal muscle of mice at all developmental stages，and its expression level gradually decreased with age. Immunofluorescence staining showed that PPAR α protein was widely distributed in the nucleus and cytoplasm of mouse gastrocnemius muscle. Further studies found that PPARα gene knockout led to a significant decrease in the expression of MyoD，MyoG and Pax7 in gastrocnemius muscle，while the phosphorylation levels of AMPK and P38 increased significantly.

Keywords： different developmental stages； peroxisome proliferator-activated receptor α （PPAR α ）； skeletalmuscle;AMPK;P38MAPK

* Corresponding authors： YAN Yi， E-mail： yanyi@sxau. edu. cn； WANG Haidong，E-mail：sxaudywhd@163.com

骨骼肌作为动物体内最大的肌肉组织，约占健康动物体重的 40% ，除承担运动与支撑功能外，亦是机体能量代谢的核心场所之一[1，并为人类膳食提供主要蛋白质来源。（剩余15969字）