EAV-HP在SLC01B3基因5'端非编码区整合影响芦花鸡肝脏代谢组学研究

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中图分类号：S852.2 文献标志码：A 文章编号：0366-6964（2026）01-0476-10

Abstract：The formation of green eggshell characteristics in chickens is associated with the high expression of Solute Carer Organic Anion Transporter Family Member 1B3（SLCOlB3） in eggshell glands initiated by the endogenous retrovirus EAV-HP. As a core metabolic organ， SLCO1B3 is highly expressed in the chicken liver. The insertion mutation of EAV-HP downregulates the expression of SLCO1B3 in the chicken liver.Metabolomics studies of chicken liver during this process have not been reported. This study took the Luhua chicken as the research object. Using the greenshelled egg genotypic primers， it was determined that the frequency of green-shelled eggs produced in 38 Luhua chickens was 47.4% .Both the integration of EAV ⋅HP+/+ and EAV- ⋅HP+/- significantly reduced the transcriptional level of SLCOiB3 in the liver of Luhua chicken （ P<0.05 ），especially the effect of the integration of EAV- ⋅HP+/+ was especially significant. A total of 8O differential metabolites were screened out by metabolomics，among which 3O were up-regulated and 5O were downregulated. The pathways through which metabolites are enriched mainly involve biological processes such as taurine and hypotaurine metabolism，the citric acid cycle（TCA cycle），carbohydrate metabolism，ether lipid metabolism and alanine，aspartic acid and glutamic acid metabolism.The research results revealed the effect of down-regulation of SLCO1B3 expression caused by EAV-HP insertion mutation on liver metabolites and metabolic pathways in chickens，providing research ideas and directions for fully understanding and utilizing endogenous retroviruses to improve the production performance of chicken flocks.

Keywords：EAV-HP；SLCO1B3;metabolomics;metabolites

∗ Corresponding authors：DING Ke，E-mail： kedingl9@163.com；CHEN Jian，E-mail： Chenilejian@163.com

蛋壳颜色是蛋的重要性状之一，主要有白色、粉色、褐色和绿色等，其中绿壳蛋因其蛋壳中的胆绿素和锌螯合物含量较高而呈现淡绿色，维生素、微量元素和氨基酸含量高，胆固醇含量低，被国家卫生部批准为“保健食品”，被誉为“蛋中人参”。（剩余13213字）