基于网络药理学和试验验证分析小檗碱抗金黄色葡萄球菌感染的分子机制

打开文本图片集

中图分类号：S853.74 文献标志码：A 文章编号：0366-6964（2026）01-0513-13

Abstract：The aim of this study was to investigate the target and and molecular mechanism of action of berberine（BBR） against Staphylococcus aureus（S. aureus） infection. This experiment first screened common targets of BBR and S ：aureus infection based on network pharmacology，constructed a PPI network，and performed GO/KEGG enrichment analysis. The binding activity of BBR and core targets was verified through molecular docking. Finally，a mouse infection model was constructed to observe pathological changes in the liver and spleen using tissue sections，and qPCR was used to validate the expression levels of the selected target genes’mRNA. The results showed that： 1） A total of 1O9 common target points between BBR and S . aureus were successfully screened. Through network pharmacology analysis，25l GO entries and 62 KEGG entries were ultimately obtained， mainly enriched in the MAPK，PI3K-Akt，and GnRH pathways. Molecular docking results showed that BBR had good binding energy with six proteins，including Casp3 and Jun，among the intersection targets.2） BBR can alleviate liver tissue lesions caused by S .aureus infection；at the same time，it can significantly upregulate the expression of Casp3 mRNA and Jun mRNA in mouse liver. In summary，this study preliminarily identified Casp3 and Jun as potential targets for BBR's anti- S aureus activity. Additionally，a mouse model of S . aureus infection was established to further confirm that BBR can alleviate liver and spleen lesions by regulating the mRNA expression of Casp3 and Jun. The findings of this study provide a theoretical basis for the development and application of BBR as an anti-S. aureus infection drug.

Keywords： Staphylococcus aureus； berberine； network pharmacology； molecular docking；animaltesting

∗ Corresponding author： YANG Li， E-mail： 563289492@qq. com

金黄色葡萄球菌（Staphylococcusaureus，S.aureus）是一种革兰阳性菌，该菌可通过皮肤、呼吸道、血液和伤口等途径感染宿主，引发乳房炎、心肌炎、脓疮等多种疾病1，是危害较为严重的人兽共患病之一[2]

目前，针对 S ：aureus感染的临床治疗仍以抗生素为主[3]。（剩余17112字）