一株牛源福氏志贺菌的分离鉴定及全基因组测序分析

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中图分类号：S852.61 文献标志码：A 文章编号：0366-6964（2026）01-0378-14

Abstract： In recent years，diarrhea in catte has been spreading in catte farms with a high mortality rate，causing economic losses. This experiment aims to isolate and identify Shigella flexneri，which causes diarrhea in a Beijing cattle farm，and to clarify the biological characteristics and genomic features of the pathogen's serotype，drug resistance，and pathogenicity. In this study， fecal samples from diarrheal catle were collcted for bacterial isolation and purification，l6S rRNA amplicon sequencing analysis，virulence gene detection，and whole-genome sequencing of isolated strains. The results showed that one strain of Shigella flexneri Fc was identified through bacterial isolation and purification，and PCR identification. Whole-genome sequencing revealed that the Shigella flexneri Fc strain has a genome size of 4.98Mb and a GC content of 50.76% . It contains 91 tRNA genes，7 23S rRNA，7 16S rRNA，8 5S rRNA，283 misc_RNA，and 97 carbohydrate-active enzyme genes. TVBD annotation analysis identified 1 363 transporter genes; CARD annotation analysis identified 80 resistance genes；VFDB and PHI-base annotation analysis identified 1 41O potential virulence factorrelated genes and 2 O52 phenotypic mutation genes. Phylogenetic tree analysis revealed that Shigella flexneri is closely related to Shigella flexneri 299O3，belonging to the same branch. This study completed the isolation and identification of a bovine-origin Shigella flexneri strain and whole-genome sequencing analysis， providing a theoretical basis for the prevention and control research of shigellosis.

Keywords： Shigella flexneri;whole-genome sequencing;drug resistance;virulence Corresponding author：WANG Zhen，E-mail： wangzhen3355@163.com

志贺菌是临床上最常见的肠道病原体之一，主要分为4种血清群：痢疾志贺菌、福氏志贺菌、鲍氏志贺菌和宋内志贺菌。（剩余17247字）