常山散对感染不同发育阶段球虫雏鸡盲肠机械屏障的影响

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中图分类号：S859.795 文献标志码：A 文章编号：0366-6964（2026）01-0526-14

Abstract：The aim of this study was to investigate the effect of Radix dichroa powder （RDP） on intestinal mechanical barrier at diferent developmental stages of chicks infected with coccidia.The experiment was divided into 6 groups，which were administered on the day before the chalenge，the day of the attack，and the 2nd，3rd，4th and 5th days after the attck.Each experimental group included Group I（Healthy control group），Group II（Infection control group） and Group II（RDP recommended dose group）.At the end of the administration，the chick cecum of each group were collected to make pathological sections. The immunohistochemical analysis of intestinal tight junction proteins occludin and claudin-1 were performed by Pro PLUS 6.O image software，and the secretion of intestinal goblet cels and mucin were determined. The results showed that compared with Group I， the expression levels of occludin protein，claudin-1 protein，and intestinal mucin in Group I and Group II were significantly reduced（ .-0.05 ），and the number of goblet cells was significantly decreased （ .-0.05 ）. Compared with Group I ，the expression of the above three proteins of Group II were significantly improved （ P<0.05 ），and the number of goblet cells were increased from the day before the challenge to 3 days after the challenge. However，with the continuous development of coccidia in chicks and the delay in administration time，the above three proteins in Group I and Group Il were markedly decreased on the 4th and 5th day of challenge （ P>0.05 ），and the goblet cells disapeared. In summary，RDP can reduce the damage of coccdia to the host intestinal tract， improve the secretion of intestinal barrier protein，and reduce the damage of coccidia to the intestinal structure；the earlier the drug is administered after the challenge，the better the improvement of intestinal damage caused by coccidia.

Keywords：Radix dichroa powder;chicks；coccidia；intestine；mechanical barrier ∗ Corresponding author ： LI Chengyi， E-mail ： gslichengyi@163. com

球虫寄生于鸡肠上皮细胞，使肠上皮细胞崩解和坏死，破坏肠黏膜的完整性，而肠黏膜对于维持健康、预防组织损伤和多种疾病，以及向机体提供营养等方面至关重要[1]。（剩余13803字）