全基因组关联分析筛选大别山牛体重体尺性状相关分子标记

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中图分类号：S823.2 文献标志码：A 文章编号：0366-6964（2026）01-0181-13

Abstract： The objective of this study was to identify molecular marker loci and candidate genes associated with body weight and size traits in Dabieshan cattle，thereby elucidating their genetic mechanisms.A total of 515 healthy Dabieshan cattle aged between 3O and 36 months were selected， phenotypic measurements of eight traits were performed，including body weight，withers height，body slanting length，chest circumference，abdominal circumference，cannon bone circumference， hucklebone width，and hip width. Genotyping was performed using the Neogen 1OoK SNP chip，and a genome-wide association study（GWAS） was conducted using a mixed linear model （MLM）. The results revealed 44 single nucleotide polymorphisms （SNPs） loci significantly associated with body weight and size traits，which were annotated to 426 candidate genes. Among these，6 candidate genes （WFIKKN2，MMP2，MATN1，PUM1，VKORCl，and VKORClL1） related to muscle development， chondrogenesis，and bone metabolism were of particular interest. Functional enrichment analysis using Gene Ontology（GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） further indicated that the VKORCl and VKORCiL1 genes are involved in key biological processes such as vitami K metabolism，which warrants further investigation. In conclusion，the findings of this study provide clear molecular targets and valuable insights for the genetic improvement of the Dabieshan cattle.

Keywords： Dabieshan cattle; SNP; body weight and size traits;whole-genome association study （204号 ∗ Corresponding authors： WANG Shujuan，E-mail： wangshujuan2Ol2@hotmail. com; XU Lei，Email： xuleirock@163.com

大别山牛属役肉兼用型，原产于大别山周边地区和长江中下游，是安徽省被列入国家畜禽品种资源志的优良地方黄牛品种[1-2]。（剩余15570字）