藏猪源产气荚膜梭菌的分离与耐药状况分析

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中图分类号：S852.616.3 文献标志码：A 文章编号：0366-6964（2026）01-0392-09

Abstract：The aim of this study was to understand the epidemic characteristics and drug resistance of Clostridium perfringens in Xizang's Tibetan pig population，and to provide theoretical basis for disease prevention and control. In this study，273 Tibetan pig fecal samples colected from 9 districts and counties in Xizang from 2O2l-2O24 were isolated and identified by bacterial isolation and purification，PCR identification，drug sensitivity test，and drug resistance gene detection and other methods，and the toxin typing and drug resistance phenotype were determined. The results showed that a total of 180 strains of C . perfringens from Tibetan pigs were obtained，with an isolation rate of 65.93% （180/273）；According to toxin gene testing，all strains were identified as type A C ， perfringens，with 142 strains containing the cpb2 gene. The results of antibiotic susceptibility tests showed that the isolated strains had high resistance to 6 drugs，including gentamicin，streptomycin， erythromycin，polymyxin B，sulfamethoxazole，trimethoprim. 97.22% （175/180）of the isolated bacteria were multidrug-resistant strains. The proportion of strains resistant to 4-6 types of antibiotics is 71.67% （129/18O）. In addition，the isolated strains carried 12 resistance genes，with a detection rate of 23.08% （12/52）. The detection rate of the aminoglycoside resistance gene aadA was the highest，with a detection rate of 85.00% （153/18O）． This study clarified the prevalence of C perfringens from Tibetan pigs in Xizang，providing data support for taking efective prevention and control measures.

Keywords： Tibetan pig; Clostridium perfringens； antibiotic sensitivity test； antibiotic resistancegenes；epidemiological investigation

∗ Corresponding author： SHANG Peng，E-mail：nemoshpmh@126.com

产气荚膜梭菌（Clostridiumperfringens）属于厚壁菌门（Firmicutes）梭菌属（Clostridium），曾称魏氏梭菌或产气荚膜杆菌，具有分布广泛且难以彻底清除的特点，可长期存在于与动物生活相关的环境、物品和自身消化道中。（剩余13525字）