重构枯草芽孢杆菌的木糖代谢通路生产乙醇酸

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摘 要：本研究旨在获得一株可利用麸皮生产乙醇酸的食品安全菌。通过同时过表达xdh和yjhG，构建了基因工程菌XDH-YjhG，该菌株能够利用麸皮生产乙醇酸，产量为3.15 g·L-1。本研究首次证明了可利用麸皮作为碳源生产乙醇酸，为乙醇酸的工业化生产奠定了基础。

关键词：枯草芽孢杆菌；麸皮；乙醇酸

Reconstructing the Xylose Metabolic Pathway of Bacillus subtilis to Produce Glycolic Acid

JI Minghua

（Kinry Food Ingredients Co.， Ltd.， Shanghai 200000， China）

Abstract： This study aims to obtain a food-safe bacterium capable of utilizing wheat bran to produce acetic acid. By co-overexpressing xdh and yjhG， a genetically engineered strain XDH-YjhG was constructed， which could use wheat bran to produce acetic acid with a yield of 3.15 g·L-1. This study is the first to demonstrate the use of wheat bran as a carbon source for the production of acetic acid， laying the foundation for the cost-effective industrial production of acetic acid.

Keywords： Bacillus subtilis; wheat bran; acetic acid

乙醇酸（HOCH2COOH）是最簡单的α-羟基酸，别名羟基乙酸或甘醇酸，可广泛用于食品、医药和化妆品等领域。（剩余3502字）