一测多评测定川银花（细毡毛忍冬） 标准汤剂6种化学成分含量

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【中图分类号】R248.1 【文献标志码】A 【文章编号】1007-8517（2026）03-0056-10

DOI：10. 3969/j. issn.1007 -8517.2026. 03. zgmzmjyyzz202603012

Determination of Six Components in Standard Decoction of Lonicera Flos （Lonicera Similis Hemsl）Through QuantitativeAnalysisof Multi-componentswith SingleMarker（QAMS）

GU Qinying ZHANG Yujing GU Chao ZHANG Yuntian ZHOU MinGUO Xingyu LI Song ZHAI Yanjuan* Jiangyin TianjiangPharmaceutical Co.Ltd.，Jiangyin214434，China

Abstract：ObjectiveToestablishamethodforthecontentdeterminationofsixcaffeoylquinicacidscomponentsinstandarddecoctionofLoniceraFlos（LonicerasimilisHemsl）throughquantativeanalysisofmulti-componentswithsinglemarker（QAMS）. MethodsFifteebatchesofLoniceraFlos（Lonicerasimilisemsl）wercollctedfrodiferethabitatsaordingtodifrentteical requirements， fifteen standard decoction samples were prepared.Poroshell 120 SB C18 column （Agilent， 4.6mm×150r nm， （204号 2.7μm ）were used，acetonitrile -0.2% phosphoric acid aqueous solution was used as mobile phase， gradient elution， flow rate was 2 0.7mL/min ， column temperature was 30qC ，and detection wavelength was 327 nm，3， 4- dicaffeoylquinic acid was chosen as the internaleferencesubstances，therelativecoectionfactors（RCFs）ofneochlorogenicacid，chlorogenicacid，cryptochlorgenic acid，3，5-caequiccin4，5cafquccideeestablishdieentcoutmpatures，foate，columsandUPLCequipmentwereusedtoinvestigatetherelativecorrectionfactorandrelativeretentiontimedurabilityTheexteral standard methodandQAMSwereusedtosimultaneouslydeteminethemassfractionsofsixcomponents infteenstandarddecoctionof LoniceraFlos（LonicerasimilisHemsl），andthediferenesbetwenthetwodeterminationresultswerecompared.ResultsTerepro ducibilityofthestablishdrelaivecorrtionfactorasgood，thereasnsgnifiantdiffreebetweenthecalculatedvauesofsix components infteenstandarddecoctionofsamplesbytheQAMSandthemeasuredvaluesoftheexternalstandard method.ConclusionThemethodestablishedinthisstudyisfast，acurateandreproducible，andcanprovidebasisforthequalitycontrolevauationof

standarddecoctionofLoniceraFlos（LonicerasimilisHemsl）.

Keywords：LoniceraFlos（LonicerasimilisHemsl）；StandardDecoction；QuantitativeAnalysisofMulti-componentswith Single Marker（QAMS）；NeochlorogenicAcid;ChlorogenicAcid；CryptochlorogenicAcid;3，4-dicafeoylquinicAcid；3，5 dicaffeoylquinic Acid；4，5-dicaffeoylquinic Acid

川银花为忍冬科植物细毡毛忍冬Lonicerasimil-isHemsl.和淡红忍冬LoniceraacuminateWall.的干燥花蕾或带初开的花。（剩余7364字）