玛卡多糖脱色工艺优化及其抗氧化活性研究

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中图分类号 R284.2

文献标志码 A

文章编号 1007-7731(2026)08-0077-07

DOI号 10.16377/j.cnki.issn1007-7731.2026.08.019

Research on optimization decolorization process of maca polysaccharide and its antioxidant activity

Liang Huiqing Liao Yuxing Zhang Cuixiang Chen Guiyuan Li Xueying (School of Basic Medical Sciences, Dali University, Dali 671000, China)

Abstract To optimize the decolorization process of polysaccharides from black maca and evaluate their antioxidant activity, this study used D101 macroporous adsorption resin as a decolorizing agent and optimized the decolorization process by response surface methodology. First, single-factor experiments were carried out to investigate the effects of resin dosage, decolorization time and decolorization temperature on the decolorization rate and retention rate of polysaccharides. On this basis, a regression model was established using Box-Behnken design. DPPH and ABTS free radical scavenging assays were performed with VC as the positive control. The absorbance values of maca polysaccharides before and after decolorization were measured and their free radical scavenging rates were calculated to compare and analyze their antioxidant effects. The results showed that the regression model was significant (P2=0.986 6 ), which could effectively predict the process parameters. The optimal process conditions obtained were as follows: crude polysaccharide concentration of 5.0 mg/mL, resin dosage of 2.0 g, decolorization time of 1.5 h, and decolorization temperature of 50 °C. Verification experiments showed that under these conditions, the decolorization rate of polysaccharides reached 55.4%, the retention rate was 53.56%, and the comprehensive score was 54.48%, which were basically consistent with the predicted values of the model. Antioxidant activity tests indicated that maca polysaccharides exhibited favorable scavenging capacity against DPPH and ABTS free radicals in a distinct dose-dependent manner. The decolorized polysaccharides still maintained satisfactory antioxidant activity, especially in ABTS free radical scavenging. In conclusion, the decolorization process established in this study was stable and feasible, and provided a reference for the purification of black maca polysaccharides.

Keywords maca polysaccharides; macroporous adsorption resin; response surface methodology; decolorization process; antioxidant activity

玛卡(Lepidium meyenii Walp.)为高原作物，其主要活性成分包括多糖、生物碱、多酚、甾醇及皂苷等，其中玛卡多糖(Maca polysaccharide, MCPC)为主要研究热点。（剩余12214字）