瑶药上山虎水提取工艺研究及3个成分含量测定

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【中图分类号】R284.2 【文献标志码】A 【文章编号】1007－8517（2026）02－0057-06

DOI：10. 3969/j. issn. 1007 -8517. 2026. 02. zgmzmjyyzz202602011

Studyon WaterExtraction Processand Determination of Three Active Componentsin YaoMedicinePittosporumpauciflorum

QIN Dan’2GAO Jie1CAO Jiajie1，²TANG Qingchen1，2 FU Peng1，2.3* 1.Guangxi University of Chinese Medicine，Nanning 5302OO，China；2.Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine，Nanning 53020O，China；3.Guangxi Medical College，Nanning 530023，China

Abstract：ObjectiveToestablishamethodfordeterminingthetotalflavonoidcontentintheYaoMedicinePitosporumpaucifl rumsystematicallyinvesigate teateretrctionprocsofPitospompaucifom，sreeaneftandtableeactoo， andquantifythecontentsofneochlorogenicacid，chlorogenicacid，andsyrigaldehydeinitswaterextract.MethodsSinglefactor experiments and an L9（33） orthogonal testdesign were employed，withdry extractyield and totalflavonoid content as evaluation indices，tooptimizetheliquidto-materialratio，extractiontie，andxtractionfrequency.Hgh－performanceliquidchromatograpy （HPLC）wasused todeterminetheconcentrationsof neochlorogenicacid，chlorogenicacid，andsyringaldehyde.Results Theoptimalextractionconditionswereidentifidasthreecyclesofoilingwith1-foldwaterfor4OminuteseachThecontentsofnhloo genic acid，chlorogenic acid，and syringaldehyde in the Pitosporum pauciflorum water extract were determined to be 0.3789mg/g ， （20 1.7608mg/g ，and 0.0646mg/g ，respectively.Conclusion This study established，forthe first time，a method for quantifying total flavonoidsinPitospompuciflomndtiedable，twatrextractiopross.dditioally，tecotentsofcl rogenicacid，chlorognicacid，andsyingaldehdeinPitosporumpucifloumweresucsfulldeterind，providingafodation for further research and quality control of this Yao Medicine.

Keywords：Pitosporum pauciflorum；Total Flavonoids；Extraction Process；HPLC Content Determination

瑶药上山虎为瑶药“五虎”之一，在广西地区资源丰富。（剩余7106字）