丹参酮IA对玻璃化冷冻小鼠卵母细胞保护机制的研究

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[关键词］卵母细胞；玻璃化冷冻；丹参酮ⅡA；水通道蛋白3；细胞内冰晶；渗透性 [中图分类号]R285.5 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2026.01.006

[Abstract]ObjectiveToinvestigatetheprotectivemechanismof TanshinoneIAagainstvitrication-inducedinjuryin mouseoocytes.MethodsFemaleICRmiceaged4-8weeks（SPFgrade）wereintraperitoneallinjectedwith5IUof pregnantmare serumgoadotropin PMG）After48oursmorphologicalloalimatrecytesat teerinalvesicle（Gstagewerel fromtheovaries.TheGV-stage oocytes were divided intocontrol groupandTanshinoneIIA groupandculturedovernight for16- 18 hours to reach the metaphase I（MI） stage. Morphologically normal MI oocytes were then subjected to in vitro fertilization， and ferilization，cleavagendblastocystfationereservdiachrou.eproteinexpresionlevelofquaporin3（Q3）on the cell membrane was detected in MII oocytes from the control group and the Tanshinone llA group using immunofluorescence technique.Inanotherexperiment，GV-stageoocytesweredividedintofourgroupsforcultureuntilmaturation：acontrol group（onvitifed），Tsooviid，rolp（vriddsop（vridFlit cleavagerate，andblastocystformationratewereobservedandanalyzedinthefourgroups.Acryomicroscopewasemployedto observetheeffectofTanshinoneIIAonintracellaricefrmation（IFduringtecropreservationofMoocyesResultsompared withtheontrolgroupthTnshinoeIAgoupshowedincreasedratesofcytematuratiofertilizationleavage，ndlastot formation （ P<0.05 ）， with the highest blastocyst rate observed when treated with O.5μg/mL Tanshinone IIA. The protein expression level of AQP3 was significantly higher in the Tanshinone llA group than in the control group （ P <0.0001）.Compared with the controlgroupovifd）soeIouovriedodsiftlyrertoeagndstot formation rates （ P O.05）.Similarlycomparedwiththecontrol group（vitrified），theTanshinoneIAgroup （vitrified）alsoexhibited significantly increased fertilization， cleavage， and blastocyst formation rates （ P- <0.05）.Additionally， IIF occurred at a lower temperature withamoreoncentratedtemperaturerange，resultinginalowerprobabilityoficeformationduringcolingConclusionTashone IA improvesthesurvivalrateanddevelopmentalpotentialofvirifiedoocytesbyupregulatingtheexpressonofAQP3onthe oocyte membrane and reducing the temperature of IIF during cryopreservation.

[Keywords] oocyte; vitrification; Tanshinone IIA; AQP3; intracellular ice crystals; permeability

据2021年国家统计局的数据报道，我国育龄夫妇不孕不育率已上升至 12%～18%[1] 。（剩余16519字）