不同植物生长调节剂对辣椒子叶外植体离体培养的影响

打开文本图片集

中图分类号：S641.3 文献标识码：A 文章编号：1006-060X（2026）01-0072-05

Abstract：ThisstudyusedthepepperinbredlineZY24astheexperimentalmaterialtoinvestigatetheeffectofdierenttypesand concentrationsofplantgrowthregulatorssupplementedintheMSmediumontheinvitrocultureofcotyledonexplants.Theresults showed that during callus induction， the 2.0mg/L 6⋅BA+0.5mg/L IAA treatment had the best effect， achieving a callus induction rate of 94.56% .During callus proliferation， 0.1mg/L 2，4-D treatment significantly promoted the proliferation of callus，while maintaining compact callus texture with a low risk of bud inhibition.During adventitious shoot elongation， the 4.0mg/L 6.BA+0.5mg/LGA3+ 0.3mg/L IAAtreatment efectively promoted adventitiousshot elongation，producing the highest numberof elongatedadventitious shoots perbud-bearingcalls，withanaverageof2.09shots.Adventitious shootcoulddeveloroot normallyonth1/2 MSedium withoutgrowthegulator.Thestudydeterminedthe typesandconcentrationsof growthregulatorsfortheinviroregenerationof pepper cotyledon explants.

Key words： pepper; cotyledon explants; plant growth regulators

辣椒（CapsicumannuumL.）起源于中南美洲热带地区，为茄科辣椒属一年生或有限多年生草本植物。（剩余7650字）