不同预处理对南荻纤维素酶解特性的影响

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中图分类号：TQ353.2 文献标识码：A 文章编号：1006-060X（2026）01-0066-06

Abstract：Thisstudyaimstorevealthemechanismbywhichformicacidcombined withalkalinehydrogenperoxideforpretreatment ontheenzymatichydrolysisefciencyofMiscanthuslutarioripariacellulose.Thephsiochemicalpropertiesandcelulaseadsoption characteristicsofdiferentpretreatedsampleserecomprehensivelyaalyedintesofforylgoupotent，Zetapotetial，nd X-ray photoelectronspectroscopy（XPS）.Theresultsshowedthat thepretreatmentwith formicacidandalkalinehydrogenperoxide decreased the electronegativity of M. lutarioriparia cellulose and attenuated the electrostatic repulsion with cellulase，thus significantly increasing the adsorption rate ofcellulase.The adsorption rate of cellulase on M. lutarioriparia cellulose1 separated by formic acid was the highest， reaching 56.86% ，and that on M. lutarioriparia cellulose 2 obtained by further treatment with alkaline hydrogen peroxide remained high （54.31% ）.XPS results showed that the main component adsorbing cellulase was lignin for M. lutarioriparia straw， lignin and cellulose for M. lutarioriparia cellulose 1，and cellulose for M. lutarioriparia cellulose 2.The conversion rate and glucose concentration of M. lutarioriparia cellulose2 were the highest among the three materialsafter 24h ofenzymatic hydrolysis， reaching 68.84% and 65.29g/L ，respectively.However， the enzymatic hydrolysis of M. lutarioriparia cellulose1 was restricted due to formylationand thenon-productiveadsorptionofligninoncelulase.Overall，the pretreatmentbyformicacidcombined with alkaline hydrogen peroxide effectively enhanced the enzymatic hydrolysis eficiency of M. lutarioriparia cellulose by reducing electronegativity， purifying cellulose， and decreasing ineffective adsorption.

KeyWords：Miscanthus lutarioriparia;cellulose;enzymatic hydrolysis;formicacid;alkaline hydrogenperoxide

南荻（Miscanthuslutarioriparius）是我国特有的禾本科芒属草本植物，适应性强、生物量大且纤维素含量高，被认为是优质的能源植物[1-2]。（剩余10724字）