台湾金线莲S-腺苷甲硫氨酸合成酶基因克隆及生物信息学分析

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中图分类号：Q753 文献标识码：A 文章编号：1006-060X（2026）01-0022-07

Abstract：To explore the functionof the S-adenosylmethionine synthetase （SAMS）gene，this studyamplified theopen eading frame（ORF）of S-adenosylmethioninesynthetase genefromAnoectochilus formosanus （AfSAMS）byPCR based ontranscriptome sequencing.Furthermore，bioinformaticstoolswereusedtoanalyzethephysicochemicalcharacteristicsoftheamplifedfragmet.The results showed that the ORF of AfSAMS was 1 191 bp in length， encoding a peptide composed of 396 residues. The putative protein had a molecular formula of C1898H2968N508O601S16， a molecular weight of 43 032.45 Da， a theoretical isoelectric point of 4.97，and an instabilityindex of25.39whichindicatedastableprotein.Theproteinhadanaliphaticaminoacidindex of80.96，conforming tothe structural characteristics of intracellular stable enzymes.The grand average of hydropathicity （GRAVY） was -0.246 ，which indicated a hydrophilic protein. The a -helixes， β -sheets， β -turns，and random coils in the secondary structure of theprotein accounted for 35.61% 15.15% 6.82% ，and 42.42% ，respectively. The predicted tertiary structure of the protein was highly similar to those of Dendrobium catenatum （XP_020681927.1） and Canna indica （WOK96063.1）.

Key words：S-adenosylmethionine synthase;gene cloning; bioinformatics;Anoectochilus formosanus

台湾金线莲（Anoectochilusformosanus）为兰科（Orchidaceae）金线兰属（Anoectochilus）植物，别名台湾银线兰，主要产于我国台湾地区，多分布在海拔 500～1600m 的阴湿森林或竹林内[1]。（剩余9314字）