黄芪及制剂中LPCR分子鉴定方法的建立

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关键词LPCR；黄芪；制剂；分子鉴定方法中图分类号R284.1 文献标识码A文章编号 0517-6611（2026）03-0176-06doi：10.3969/j. issn.0517-6611.2026. 03. 033开放科学（资源服务）标识码（OSID）：

Establishment of LPCR Molecular Identification Method for Astragalus membranaceus and Its Preparations TIANYan-qu，HUANLinge'，AZhaoyang²etal（1.BologicalEngineringLabratoryScholofPhaacyHunanUity of Chinese Medicine，Changsha，Hunan42O8;2.HunanDanjianPharmaceuticalTechnologyCo.，Ltd.，Changsha，Hunan4100）

AbstractObectie]TevelopamolecularidenificationmethodforAstragalusmembranaceusanditsphamaceuticalpreparatiosusing ligation-dependentPCR（LPCR）technology.Method]Basedonsinglenucleotidepolymorphism（SNP）locispecfictoAstragalusmembranaceus，hybridzationpobsndCRprisesigdndeteionosresstematicalltidtaateth's specificity.ThearoachasfurthervalidatedusingcommercialsamplesofGuhanHealthEseceandZhenqiFuzhengGranules.esulte establishedLededgiidyrescdplsfulledild ples，includingrawAstragalusmembranceusmaterials，GuhanHealthEssence，ndZenqiuzhengGaulesElectroporeticalyiso fimedtheprseceofssspecifcdsespdigtstrglusmbacs.otablyactiviybedwih monlyconfusedspeciessuchasHedysarumpolybotrysandngativecontrolslackingAstragalusmembranaceusDNAremainedudetected. [Conclusion]TeLPCRmethodevelopedinthisstudyenablespreciseidentficationofAstragalusmembranceusDAinomplexhrbal prepartionsandisparticularlyefectiveforsamplescontaininghiglydgraddDA.Tisapproachprovidesareliableandobustoolfor quality control and regulatory monitoring of traditional Chinese medicine products.

KeyWordsLPCR;Astragalus membranaceus; Preparation;Molecular identification method

黄芪（Astragalusmembranaceus）作为我国传统中药沿用至今，并已载入《中华人民共和国药典》，其具有补气升阳、固表止汗等作用，已成为许多中成药（traditionalchinesepatentmedicines，TCPMs）和中药制剂的重要成分之一。（剩余8738字）