苏打盐碱胁迫下东北主栽大豆萌发期耐盐碱性综合评价模型构建及生理机制研究

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关键词大豆；苏打盐碱胁迫；萌发期；耐盐碱性；综合评价；主成分分析；生理机制

中图分类号S529文献标识码A

文章编号 0517-6611（2026）03-0030-05

doi ： 10.3969/j . issn. 0517-6611.2026. 03. 006

开放科学（资源服务）标识码（OSID）：

ComprehensiveEvaluationofSoybeanTolerance toSoda Salineg-AlkalineStressat Germination StageandScreeningofEliteGerplasm in Northeast China PENG Hao，TANCheng-you，YANGJunetal（PeanutResearch Institute，Baicheng Academyof AgriculturalSciences，Baichengilin ）

AbstractTodressthhallngeosedbysalialkalisoilsiestritisobeanproductioninNrthastCathiitevelop anobjectiveandcomprehsiveevaluatiosystemfoasessngsobeansaltalkalitoleraeuringtegeationstage，sresuperior germplasms，dudateudlgslalss，tebpdingtheticalsupprtfohdgflaole antsoybeanvarieties.Using43majorsoybeancultivarsfomNortheastChinaasmaterials，germinationeerg（GE），germinationpcentage （GP），germination index（GI），radicle Na+/K+ ratio，and antioxidant enzyme （SOD，POD）activities were measured under simulated soda saline-alkali stress [pH9.0 dS/m].A comprehensive tolerance index （CTI）model was constructed by integrating one-way analysisfvariance（ANOVA），K-meanslustering，ndpricipalomponentanalysis（C）eesultssoedthatallasurdtaits exhibited extremely significant differences （ P<0.01 ）among cultivars under stress. K-means clustering categorized the cultivars into highly tolerant（6），moderatelytolerant（27），andsensitive（1O）types.TheCTI model，basedonPCA（cumulativecontributionrate 84.51% ）， showed high consistency with the clustering results （accuracy 92.7% ，Kappa coefficient=0.87）. Highly tolerant cultivars （“Jiyu 4520”） exhibited superior performance ： germination percentage >85% ，radicle Na+/K+ ratio <0.40 ，and SOD and POD activities higher than 186 and 36U/（g⋅min） ，respectively.TheCTImodel efectivelyevaluates soybeansalt-alkali tolerance atthegermination stage；maintaining ion homeostasis （manifested as a low radicle Na+/K+ ratio）and activating the antioxidant defense system are key physiological mechanisms for highly tolerant cultivars to cope with soda saline-alkali stress.

KeywordsSoban;Sodasalikalinestres;Geationsage;Salialalinetolerae;Compreesievauatio;ripalcopoanalysis（PCA） ;Physiological mechanism

土壤盐渍化是全球范围内威胁农业生产和粮食安全的重要非生物逆境因素。（剩余7229字）