基于GBS技术的东北刺五加群体遗传分析

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关键词刺五加;GBS;单核苷酸多态性（SNP）;遗传结构;遗传多样性

中图分类号S567. 1+9 文献标识码A

文章编号 0517-6611（2026）03-0166-05

doi：10. 3969/j. issn. 0517-6611. 2026. 03. 031

开放科学（资源服务）标识码（OSID）：

Population Genetic Analysis of Eleutherococcus senticosus in Northeast China with Genotyping by Sequencing（GBS）

LI Xiang-quan，LIUYun-wei，AIZqiangetal（YichunBranchofHeilongjangAcadeyofForestry，chun，Heilongjiang00） AbstractOectieTfcielpotctteeticsousofEleterococseticossgeplasodUsingotb quencing（GBS） technology，single-nucleotide-polymorphism（SNP） site mining were identified in 120E .senticosus individuals from four populationinNtastCinandthrgeticructureadgeticdivesityeaalyd.esultoplatioevolutioarytaly sis，principal component analysis and structureanalysis，combined with the geographical distribution characteristics of E . senticosus， four populations of E .senticosus wereclusteredintothree diferent genetic groups.Butthe geneticboundariesbetween populationswerenotobvious， there were some degres of gene exchange. Analysis of the 103 546 high-quality SNPs exposed that populations of E . senticosus in Northeast China had lower genetic diversity （Private =1 943.25， π=0 239， Ho=0 .2353， He=0 . 234 3），there were close genetic similarities and little genetic diferences among different groups，only moderate differentiation exists between Jilin and Liaoning（ Fst=0 .0532），indicating that the genetic basis of E .senticosus in Northeast China wasrelatively narow，whichmaybedue toitsclonalreproductive characteristics.[Coclusion]The results not only provide molecular tools for genetic studies of E .senticosus， but also contribute to germplasm conservation and utilization in Northeast China.

KeywordsEleutherococus senticosus ;GBS;Single-nucleotide-polymorphism （SNP）;Genetic structure;Genetic diversity

刺五加（Eleutherococcussenticosus）系五加属（Eleuthero-coccus）多年生灌木，分布于我国东北3省（黑龙江省、吉林省、辽宁省）河北省、山西省，是我国珍贵药用植物，列入《中华人民共和国药典》（2020版）[1]。（剩余8258字）