PBK/TOPK基因对三阴乳腺癌MDA-MB-453细胞株化疗药物敏感性的影响

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中图分类号：R736.3 文献标识码：A文章编号：1006-1959（2026）03-0008-06

DOI：10.3969/j.issn.1006-1959.2026.03.002

Effect of PBK/TOPK Gene on Chemosensitivityof Triple Negative Breast Cancer CellLineMDA-MB-453

LIUYuhong1，LIUTao²，JILe²，WANGYanmei³，LIXiaoyong²

（1.DepartmentofRheumatology，theAffiliatedHospitalofYan'anUniversity，Yan'an76Ooo，Shaanxi，China;2.DepartmentofGeneralSurgery，theAfiliatedHospitalofYan'anUniversityYan'an716ooo，Shaanxi，China;3.DepartmentofHistologyandEmbryology，Medical CollgeofYan'anUniversity，Yan'an16O99，Shaanxi，China）

Abstract：OjetiTestigateeofDbngseBAKcratedproteinaseOPnoii oftriplenegatiebeasaecelle-MB43disoelaeeasmMehdsOPKNAastraseedintDB-453 cellsbylentiviustrasfetiotcheReal-tifuoresequantitatieCRasedtoeenteOPK（sOK）withteoout effect，ndtheol（opstupCellrachsasedtoetecellgratindasD-45lihop were treated with different concentrations of paclitaxel，docetaxel and 5-fluorouracil for 72h ，and cell proliferation was detected by MTT assay. ResultsTOPwihsttefeetaseleecellasstnd4mpaediho the MDA-MB-453 cell migrationabilityof the si-TOPK group was significantly reduced（ P<0.01） .After treatment with diferent concentration of palitaxeleaedacilistiol proliferation of si-TOPK group was significantly down-regulated（ P<0.01） .Conclusion TOPK gene can effectively decrease the sensitivity of human breastcancereleapeclieleldouacilced strategy forbreast cancer treatment.

KeyWords：PBK/TOPK gene;Human breast cancer cells; Lentivirus; Chemosensitivity

三阴乳腺癌（triplenegative breast carcinoma，TNBC）是指ER、PR和HER2均阴性的特殊乳腺癌，占乳腺癌的 10%～23.8% ，其恶性程度高。（剩余9188字）