防城金花茶SSR遗传多样性分析

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关键词：防城金花茶（Camellia nitidissimavar.phaeopubisperma）;遗传多样性;SSR;遗传结构中图分类号：S685.14;Q943.2 文献标识码：A

文章编号：0439-8114（2026）01-0097-08

DOI：10.14088/j.cnki.issn0439-8114.2026.01.017 开放科学（资源服务）标识码（OSID）：I

Study on the SSR genetic diversity of Camellia nitidissima var.phaeopubisperma

LI Shuang-shuang1，GAOHui'，SU Meng-xue1，CHENJin-mei，XIELei-hui²，3， HUANGYong-guang²³，LINXing，DENGJia-gang1，XIEYang-jiao

（1.GuangxiUvesityfsediceag3Oha;FgegngAsotiofrietistsndchlts， Fangchngang8ainaFgngsialadial

Abstract：ToexploretegeneticdversityofteCamellanitidissimavar.aeopubispegerlasmesoures，atotalof1mplesofCamelianitidissmavar.phaeopubispermafromthesamehabitatwithdiversephenotypictraitsereanalyzedusingSSRmolecular markers.The results showed that the 32 pairs of primers detected a total of 224 alleles （ Na ）across the 111 samples，with an average of approximately 7 allles per locus.The total number of effective aleles （ Ne ） was 95.22，and the average effective allele number was 2.976. The average values of observed heterozygosity（ Ho ），expected heterozygosity（ He ），and the Shannon index （I） were 0.475，0.562，and1.194，respectively.Theaveragepolymorphisminformationcontent（PIC）was0.528.Analysisofmolecularvariance（AMOVA） showed that 9% of the total genetic variation originated among populations，while 91% originated within populations （ 20% among individuals and 71% within individuals）.The clustering results obtained from the unweighted pair group method with arithmeticmean（UPGMA），principalcoordinateanalysis（PCoA），andSTRUCTUREanalysiswerelargelyconsistent.Allthree methodsclasifiedthe111samplesintotwogenetic groups，andtheirphenotypiccharacteristicsalignedwithpreviousfindings.This furtherconfiedtatthdiversitinleafphenotyictaitsofFnghngCamelianitidissimaascloselyaoiatedwititseetic background.

Key Words：Camellia nitidissima var.phaeopubisperma；genetic diversity；SSR；genetic structure

收稿日期：2025-07-24

金花茶（Camellianitidissima）是山茶科（Theace-ae）山茶属（Camellia）常绿小乔木或灌木。（剩余13156字）