基于流式细胞仪分析的花魔芋倍性检测条件筛选

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关键词：花魔芋（AmorphophalluskonjacK.Koch）;流式细胞仪；倍性检测中图分类号：S632.2 文献标识码：A文章编号：0439-8114（2026）01-0078-06DOI：10.14088/j.cnki.issn0439-8114.2026.01.014开放科学（资源服务）标识码（OSID）：

Screeningof ploidydetectionconditionsin Amorphophalluskonjac based on flowcytometricanalysis

LYULian-xin 1，2 ，JIAOYing-ying1，²，ZHANGShi-wan1，²，LI Xiao-qian 1，2 ， LIU Miao 1，2 ，HE Fei1，2 （1.EngineeringResearchCenterofSelenium-richKonjac，UniversitiesofShaanxiProvince，Ankang725ooo，haaniChina; 2.SchoolofModernAgriculture&Biotechnology，Ankang University，Ankang7250oo，Shaanxi，China）

Abstract：TakingtreshalltissueofAmooallusoncK.Kochsaterals，teifuencsofsoationrs centnucleiccidstains，fleringtimes，ndcentrifugationprotocolsonplidydetectionwerecompared.Theresultsshowedat，MGb lysisbuffer yielded significantly higher counts of A. konjac nucleus compared to MgSO 4 andTri s-MgCl2 buffers，witha prominent peak and minimal fragment peaksobserved.The coefficient of variation（ C V）was calculated at 4.47% .Fluorescent staining with DAPI demonstratedcomparableeficacytoP，withtheaddedadvantageofreducingbackgroundfluorescencenoisewithoutRNAasetreatment， therebyconservinganalysistime.Boththeprimaryfiltrationandthesecondaryfiltrationmethodscouldefectivelyemoveadhered cellsandcelldebris.Thesecondaryfiltrationmethodcollectedmorecellnucleiandhadabettefect.Optimaluclearsuspesion preparationinvolveddirectligtavodaneandinsitusaningpost-fltratio.TheproposedflowcyometricprotocolforA.ojacploi dyanalysis involved：homogenizing 0.25g offreshcallus tissue in1mLMGblysisbuffer，filtering，stainingwith 50μL DAPI in darkness for 3O minutes，followed by immediate analysis.

Keywords：AmorphophalluskonjacK.Koch;flowcytometry；ploidydetection

魔芋（Amorphophalluskonjac）是天南星科（Ara-ceae）魔芋属（AmorphophallusBlume）多年生草本植物[]，是惟一能大量提取葡甘聚糖的经济作物[2]魔芋葡甘聚糖属于高分子水溶性膳食纤维，凭借其优异性能在农业、食品加工、医药健康、化工及纺织产业等多个领域展现出广泛的应用前景，具有较大的商业价值[3]。（剩余7978字）