高效液相色谱法测定食品中的丙酸钠、丙酸钙含量分析

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Determination of Sodium Propionate and Calcium Propionate in Food by High Performance Liquid Chromatography

QUAN Taikang （Food Safety Inspectionand Testing Center，Dejiang CountyMarket Regulation Bureau）

Abstract：Objective：To establishahigh performance liquid chromatography method forthe determinationof sodium propionate and calcium propionate contents in food.Methods： A C18 chromatographic column was selected for the experiment.The mobile phase was methanol ⋅0.02mol/L potassium dihydrogen phosphate solution. The column temperature was constant at 30∘C ，the flow rate was 1.0mL/min ，and the detection wavelength was 214nm Samples wereseparated and detected.Results：Within therange of 0.05-1.0mg/mL ，sodium propionate and calcium propionate showed a good linear relationship， with correlation coeficients both exceeding 0.9995.The recovery rate of the method was 97.5%～104.6% ，and the relative standard deviation was less than 3% .Conclusion：This method features a simpleoperationprocess，rapiddetectionspeed，andaccurateandreliableresults.Ithassignificant effectiveness and practicality in the determination of sodium propionateandcalcium propionate in food and can be used for rapid screening and quantitative analysis ofbatch samples.

Keywords： high performance liquid chromatography; sodium propionate; calcium propionate; content determination

0引言

丙酸钠和丙酸钙作为国际公认的安全型食品防腐剂，凭借对霉菌、酵母菌及部分细菌的强效抑制作用，在食品工业中占据不可替代的地位。（剩余3947字）