发根农杆菌介导的冬凌草遗传转化体系的建立

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关键词冬凌草；发根农杆菌；毛状根；遗传转化体系；诱导率；外植体再生

中图分类号S567. 23+ 9文献标识码A

文章编号 0517-6611（2026）07-0148-04

AbstractToestablishaneficientgenetictransfoationsystemforhiryotsofRabdosiaubescens，thisstudyusedleaveandteeg mentsofRabdosiarubescensasexplants，hichwereinfectedwitAgrobactermzogenestrainsA4andG.Takingtheinductioatef hairyrootsstide，tsoftoschctealoeatioetoianttAobce genesstrainadgeeratiooceatotductitseatallesigatdulssodt est induction rate of 38.67% was achieved when stem segments of Rabdosia rubescens were infected with strain A4 for 2O minutes at a bacterial concentration of OD=0.6 ， followed by co-cultivation on a shaker at 60-70r/min for two days. The optimal concentration of cefotaxime sodium for explant regeneration was 500mg/L .PCR amplification detection confirmed that the T-DNA fragment of the Ri plasmid from AgrobacteriumrhzogeneshadeenintegratedtoteplantgenoeThissudysuessullystablisedheetictransfoatiosysteofabdosia rubescens，laying a solid foundation for the breeding of new Rabdosia rubescens varieties.

KeyWordRbdosiaubecensHensl.）Har;grobacterimzoges;；Hairyots;Geetictrasforatiosste;ductionateplat regeneration

冬凌草[Rabdosiarubescens（Henmsl.）Har]为唇形科香茶菜属植物碎米䅉的地上全草部分，别名冰凌花、彩花草等，广泛分布于南太行山山麓，具有清热解毒、活血止痛等功效[1-2]，冬凌草富含冬凌草甲素[3-4]，有很好的抗癌活性[5-6]特别是济源冬凌草，其甲素含量高于其他地区[7]，因此人们对于冬凌草的需求日益增长。（剩余8028字）