葡萄霜霉病LAMP显色法和LAMP-qPCR荧光法快速检测体系建立

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关键词葡萄霜霉菌；ITS序列；分子检测；LAMP扩增；实时荧光定量PCR

中图分类号S436.631文献标识码A

文章编号 0517-6611（2026）07-0131-03

Abstract[Objective]Establishingaapidmoleculardetectionmethodtopredict theocurrenceofdownymildewearlyMethodUsing DNAextractedfromtheifectedleavesastemplate，andLAMPprimersweredsigedacordingtotheISsequenceofdowymildewfungi. ThepathogenicfungitobeexaminedasdetectedbyolorimetricandfuorescenceofLAMP.Resulteminumdetectionliitofcolormetric LAMP was 36.5fg/μL ，and fluorescence LAMP was 36.5pg/μL ，the sensitivity was 1 000 times that fluorescence LAMP. The two methodswereconsistentwiththemorphologicalcharacteristicsoftefungus，ndthegrapeshadinfecteddowymildew.ConclusionAapid molecular detection system for grape was established which can be used for the early diagnosis of diseases.

：y word Grape downy mildew;ITS sequence;Molecular detection;LAMP amplification;Real-time PCI

葡萄霜霉病是世界范围內葡萄生产中最具挑战性的叶部病害之一[]，该病不仅阻碍了葡萄的生长发育，还影响了葡萄的质量与产量，一般年份可减产 10%～20% ，严重的可达70%～80% ，目前该病的最主要防治手段仍是喷施化学农药，但随着施药量的增加，出现了果实品质下降、环境污染、病菌抗性产生等一系列问题。（剩余5099字）