海南龙血树NBS-LRR基因家族的鉴定、密码子偏好性与表达分析

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，，，.海南龙血树NBS-LRR基因家族的鉴定、密码子偏好性与表达分析[J].江苏农业学报，2026，42（3） ：625-637.

关键词：海南龙血树；NBS-LRR；基因家族分析；密码子偏好性；表达模式中图分类号： S567.1+9;S687.9 文献标识码：A 文章编号： 1000-4440（2026）03-0625-13

Abstract：NBS-LRR is the largest gene family amongplantdiseaseresistance genes and playsanimportantrole in plantresistance and immuneresponse.Basedonthe previouslycompletedwhole-genomedataofDracaenacambodianaPiereexGagn.byourresearchgroup，thisstudysystematicallyidentifedthe NBS-LRR gene familyof D .cambodiana using bioinformatics methods，and analyzed itsphysicochemical properties，chromosome localization，gene structureandcodon preference.Theresults indicated thatthere wereatotal of 95 DeNBS-LRRcoding genesin D.cambodiana，whichwereunevenlydistributedon14outof2Ochromosomes.Thesegenesweredividedintosixsubfamiliesincluding CNL，CN，RNL，RN，NL，and N. The NBS-LRR protein of D . cambodiana was generally hydrophobic and was predicted to bedistributed inthenucleus，cytoplasm，cellmembraneandchloroplast.DcNBS-LRR proteinshadtenrelativelyconserved motifs，andmostofthemwere arangedinstableorder.Codonbiasanalysis showedthatDNBS-LRR preferredcodons ending in A/U，andthere were31highfrequencycodons，of which17wereconsidered tobeoptimalcodons.Parityrule2（PR2）-plot analysis，efective numberofcodon （ENC）-plotanalysisand neutral plotanalysisshowedthatthecodonusagepreferenceof Dc 1 NBS-LRR gene family was mainly influenced bynatural selection.Codon usage frequencyanalysis indicated that Saccharomyces cerevisiae，Nicotiana tabacum and Arabidopsis thalianacould allserveas ideal receptors for heterologous expressionof DcNBS LRR genes.Inaddition，the expression dynamicsof DcNBS-LRR genes inresponse to Fusariumoxysporum infection wereanalyzed.Theresultsshowedthattheexpresionofmost DeNBS-LRR geneswassuppresed intheearlystageandmiddlestageof infection（12h，1d，3d，and7d），whiletheexpresionwashigherinnon-infectionand3daysof infection.Tissudyisth firstto identify and analyze the characteristicsof theNBS-LRR gene familyin D cambodiana.The research resultswill provide clues for revealing the disease resistance mechanism of D .cambodiana and the molecular mechanism of Dragon’s blood formation，and lay the foundation for the subsequent in-depth analysis of the function of DcNBS-LRR.

Key words：Dracaena cambodiana Piere ex Gagn.；NBS-LRR；gene family analysis；codon bias；patern of expression

海南龙血树（Dracaena cambodiana PierreexGagn.）是天门冬科龙血树属植物，主要分布在中国沿海以及东南亚地区。（剩余14238字）