藏野驴源芽孢杆菌的分离及全基因组序列分析

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中图分类号：Q939.92 文献标识码：A 文章编号： 1000-4440（2026）03-0531-11

，，，．藏野驴源芽孢杆菌的分离及全基因组序列分析[J]．江苏农业学报，2026，42（3）：531-541.doi：10.3969/j.issn.1000-4440.2026.03.011

Isolation and whole genome sequence analysis of a Bacillus strain from Equus kiang

SONG Bo， YANG Ayan， WEN Guoqin， ZHANG Tingfu， QIN Mingsen（CollegeofLifeScience，ChinaWestNormal University，Nanchong637Ooo，China）

Abstract：ABacillus strain TEB-1 with biocontrol effcts was isolated from the feces of Equus kiang.This studyconducted whole-genome sequencingandmulti-dimensionalbioinformaticsanalysisof strain TEB-1basedonthird-generation sequencing technology.The sequencing datawere processedthrough gene assembly，geneprediction，andfunctionalannotation，within-depthminingandbiosyntheticpotentialevaluationofsecondarymetabolitesynthesisgeneclusters usingsoftware such asantiSMASH.The inhibitory effctsofstrain TEB-1against twoplantpathogenic fungi，Coletotrichum siamense and Corynespora cassicola，wereverified using the mycelial growthrate method，and drug resistance tests were performed on strain TEB-1.Theresultsshowedthat thecomplete genomeofstrain TEB-1was 4102747bp inlength，witha G+C content of 43.8% ，containing 4 O65 coding genes，85 tRNAs，and 3O rRNA genes.Bioinformatics analysis revealed abundantsecondary metabolitesynthesis geneclusters and multipledrug resistancegenes inthis strain.Functional validationresultsindicatedthatthesterilefermentationbrothofthestrainexhibitedvaryingdegreesofinhibitoryactivityagainstColletotrichum siamenseand Corynesporacassicolaat concentrations of 2.5% ，5.0%， 7.5% ，and 10.0% . Atthe 10.0% concentration，the inhibition rates reached 51.4% and 38.7% ，respectively.K-B disk diffusion tests demonstrated that strain TEB-1 was resistant to the antibioticsampicilln，penicilin，andlincomycin，whileshowinghighsensitivitytotetracycline，gentamicin，chloramphenicoleryt romycin，ciprofloxacin，cotrimoxazole，andceftraxone.TesefindingsindicatethatstrainTEB-1possessessignificantantifungalactivityalongsidecontrollbleresistancecharacteristics.Theresultsofthisstudyprovideatheoreticalbasisfordeveloping strain TEB-1 into an environmentally friendly biopesticide.

Key words：Equus kiang；Bacillus；biocontrol bacteria；whole-genome sequencing；bacteriostasis

芽孢杆菌属（Bacillus spp.）细菌作为一类具有重要应用价值的生防微生物，其广谱抑菌活性与独特的环境适应性已引起学界广泛关注。（剩余15850字）