向日葵HaOSCA1.2基因克隆及表达特性分析

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中图分类号：S565.5 文献标识码：A 文章编号： 1000-4440（2026）03-0475-10

Abstract：The hyperosmolarity-gated calcium-permeablechannel family genes （OSCA）playa key regulatory role in plant responses to abiotic stresses. To clarify the molecular characteristics of sunflower HaOSCAI.2 and its response mechanism to stresses，this study clonedthe HaOSCA1.2 gene.The coding sequence（CDS）of HaOSCA1.2 is 2322 bp in length，encoding773aminoacids.Therelativemolecular weightoftheencoded protein is8843O，withatheoreticalisoelectric pointof 8.81.Itcontains theRSN1_7TMconserved domainandisahydrophobic transmembrane protein，whosesecondary structure is dominated by α -helices.Evolutionary analysis revealed that HaOSCA1.2 shared high amino acid sequence similarity 88.93%-89.13% ）with OSCA proteins from Tanacetum cinerariifolium，Arctium lappa，and Lactuca sativa，indicating a close phylogenetic relationship.Tissue-specific expression analysis revealed that the expression level of HaOSCA1.2 in sunflower leaves was significantly higher than that in roots and stems（ P< 0.05）.Analysis of abiotic stress response showed that the HaOSCA1.2 gene exhibited rapid and upregulated expressionunder drought，high salt，and mixed saline-alkalistresses.Protein-proteininteractionpredictionindicatedthatHaOSCA1.2potentiallyinteractedwithtwomembersof the DnaJheat shock protein （HSP4O）family.This study clarifies the molecularcharacteristicsand abiotic stress response patternsof thesunflower HaOSCA1.2 gene，providinga theoreticalbasisandgeneticresources foranalyzing themolecular mechanisms of sunflower stress resistance and for breeding stress-resistant varieties.

Key words： sunflower；OSCA1.2 gene；expression characteristics；abiotic stress

由于植物具有固着生长的特性，其全生育期会持续面临多种非生物胁迫。（剩余16112字）