转抗菌肽基因Ace-AMP-sn抗纹枯病小麦新品系的 创制与评价

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中图分类号：S512.1 文献标识码：A 文章编号： 1000-4440（2026）03-0446-06

郭炜，张巧凤，祝秀亮，等.转抗菌肽基因 Ace–AMP–sn 抗纹枯病小麦新品系的创制与评价[J].江苏农业学报，2026，42（3）：446-451.

doi：10.3969/j.issn.1000-4440.2026.03.002

Abstract：Sharp eyespot has becomea major soil-borne fungal disease threatening wheat production in China in recentyears.Breedingdisease-resistantvarietiesisthemosteconomicalandefectivestrategyforcontrolingthisdisease.Inthis study，the T2 generation wheat variety Yangmai 18 transformed with the onion antimicrobial peptide gene Ace-AMP-sn was used as the material. Through successive generationsof resistanceidentification，three T，transgenic linesE308，E328，and E332 wereobtained.Quantitative real-timePCR（qRT-PCR）resultsshowedthattherelativeexpressionlevelsofAce-AMP- sn inT 7 transgenic lines E308，E328，and E332 were significantly higher thanthose intherecipient variety Yangmai 18（ P<0.05） .Results of disease resistance identification demonstrated that the sharp eyespot disease indices of the three T7 transgenic lines were significantly lower than that of Yangmai 18 （ P<0.05 ）， while no significant difference was observed compared with the resistant variety CI12633（ P>0.05 ）. These results indicated thattheintroductionofAce-AMP-sncouldefectivelyimproveresistancetosharpeyespotinwheat.Furthermore，nosignificant differences （ P>0.05 ）were detected between all T7 transgenic lines and Yangmai 18 in key agronomic traits，including heading date，plantheight，spiketraits，andyieldcomponents，suggestingthattheintroductionofthis genehadno adverse effctsonmain agronomic traits.This study provides new germplasm with outstanding resistanceand excelent agronomic traits for the genetic improvement of sharp eyespot resistance in wheat.

Key words： wheat sharp eyespot；antimicrobial peptide；transgene；resistance

小麦纹枯病（Wheat sharp eyespot）是一种由禾谷丝核菌（Rhizoctoniacerealis）引起的毁灭性土传真菌病害，广泛分布于全球主要小麦产区[1-3]，其在中国为害尤为严重[4]。（剩余10443字）