水稻萌发期温度胁迫响应的转录调控机制

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中图分类号：S511 文献标识码：A 文章编号：1000-4440（2026）03-0433-13

Abstract：Temperature isan important factor afecting rice seed germinationand yield formation.A germination experiment was conducted under three temperature treatments， including normal temperature （NT， 25‰ ），low temperature （LT， 15‰ ），and high temperature（HT， 37qC ）with Nipponbare as the test material in this study，and rice seeds cultured in dark for 60h were collected for transcriptome analysis. The results showed that compared with normal temperature treatment，lowtemperaturetreatmentcouldsignificantlydelayseedgermination，whilehightemperature treatmentaccele ratedseedgermination.Atotalof8010，3929and9427 differentially expressed geneswere identified in the low temperature and normal temperature comparison group，the normal temperature and high temperature comparison group，and the low temperature and high temperature comparison group，respectively.Results of KEGG enrichment analysis showed that temperature stress seriously disturbed the carbon and nitrogen balance during seed germination

anddestroyedtheenergyandmaterialsupplyrequired forseed germination.The planthormone signaltransductionpathway showedatemperature-specificresponsepattrn.Low temperature（LT）interfered with gibberelin metabolismandabscisic acid metabolism，whilehightemperature（HT）onlyaffcted theabscisicacid pathway.Under temperature stress，the expresionlevels of genes related to jasmonic acid signaling pathway and salicylicacid signaling pathway in germinated rice seedschanged significantly，indicating that temperature stress could activate the immune defensive response of rice seeds. Lowtemperaturecould significantlyinduceascorbicacid metabolismandaldonicacid metabolism，whilehigh temperature could promote the biosynthesis of terpenoidskeletonandubiquinoneandother terpenoidquinones.The expresionlevelsof several important transcription factor family members changed significantly.These transcriptionfactors canenhanc seed stress tolerancebyregulating the expressonofdownstream stress genes.The resultsof this studysystematicallyrevealed the transcriptionalregulatorynetworkofriceseedsinresponsetotemperaturestress，whichareof greatsignificanceforcultivating wide-adapted rice varieties.

Key words：rice； germination； temperature stress；transcriptome analysis；differentially expressed gene

稻米是中国 50% 以上人口的主粮，水稻在中国粮食生产中占据重要地位。（剩余17022字）