番茄钙依赖蛋白激酶基因CDPK6负调控植株耐热性

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中图分类号：S641.2 文献标识码：A 文章编号：1000-4440（2026）03-0606-10

翟心雨，纠敏，魏利辉，等.番茄钙依赖蛋白激酶基因CDPK6负调控植株耐热性[J]．农业学报，2026，42（3）：606-615.doi：10.3969/j.issn.1000-4440.2026.03.019

Abstract：To explore the functional diferentiationregulatory mechanisms thecalcium-dependent protein kinase（CDPK）family in tomatounderhigh-temperature stress，thisstudysystematicallyanalyzed theexpresion pattrns tomato CDPK genes under high-temperature stressusing quantitative reverse transcription polymerase chain reaction（qRTPCR）technology.Theresultsshowed that therelative expresion level CDPK6 gene was significantlyup-regulated under high-temperature stress for one hour or longer.To verify the effect the CDPK6 gene on tomato heat tolerance，virusinduced gene silencing（VIGS）technology was usedto obtain CDPK6-silenced plants，the plant phenotype relatedphysiological indices were determined under continuous high-temperature stressat .Theresults indicated that，under high-temperature stress，compared with wildtype（WT）plants，the CDPK6-silenced plants had a significantlylower heatinjuryindex relative electrical conductivity. Theactivitiessuperoxide dismutase （ SOD ） peroxidase （ P（DD ）showed a trend first decreasing then continuously increasing with the extension high-temperature stress duration.At 6h 12h hightemperature stress，the SOD activity CDPK6 -silenced plants was significantly higher than that WT plants green fluorescent protein（GFP） -silenced plants.At 24h high-temperature stress，the PD activity was significantly higher than that WT plants GFP plants.The content reduced glutathione（GSH）increased continuously with prolonged hightemperature stress， was significantlyhigherin CDPK6-silenced plants than in WTGFPplants after24h stress. The photosyntheticsystemCDPK6-silenced plants exhibited greaterstability，whichnotonlyefectivelysloweddown the increasing trend initial fluorescence （ F0 ）but also inhibited the abnormal increase maximum photochemical efficiency （ Fv/Fm ）， the OJIPcurves could stably maintain normal physiological characteristics.In conclusion，CDPK6-silenced plants exhibit significant heat toleranceadvantages，indicating thatthe CDPK6 gene has important application value in improving tomato tolerance to high-temperature stress.

Key words：tomato；calcium-dependent protein kinasegene；gene silencing；high-temperature stress；thermotolerance

近年来，在温室气体浓度持续攀升、全球气候系统不断升温的背景下，极端气候呈现多发态势，其中高温天气表现尤为突出，不仅发生频率显著上升、持续时间明显延长，极端高温纪录更是屡被刷新[1]这类极端温度会对农作物生长造成严重的非生物胁迫。（剩余14629字）