棉花纤维长度GhCRK26-1931SNP分子标记的开发及应用

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中图分类号：S562 文献标识码：A 文章编号： 1000-4440（2026）03-0464-11

Abstract：As a core indicator of cotton fiberquality， fiberlength plays a crucial role in accelerating the breding process through the development of itsmolecular markers.In thisstudy，amolecular marker（GhCRK26-1931 SNP）associatedwithcottonfiberlengthwasdevelopedvialocalization based on bulked segregant analysis combined with whole-genome sequencing（BSA-Seq），and itsapplication value was systematically evaluated.Competitiveallele-specific PCR（KASP）genotypingand phenotypic analysis were conducted on 512 coton varieties （lines）.Theresultsshowed thatthe tested materialsexhibitedrichgeneticdiversity，andthereweresignificantphenotypicvariationsinfiberlengthamong extremefibermaterialsof Gossypium hirsutum-Gossypium barbadense backcrosspopulations， G. hirsutum germplasm materials，and G barbadense germplasm materials，with the coeficient of variation ranging from 3.48% to 9.65% . Cluster analysis based on phenotypic data suchas fiberlengthdividedthe materials intofour majorgroups.Verificationtestresults indicated thatthegenotypingresultsof GhCRK26-1931 SNP molecular marker in the parental Line 9 of the G∗ hirsutum-G. barbadense backcross populations and Xinhai 16 werecompletelyconsistentwiththeBSA-seqdata：Line9hadthe TTgenotype，while Xinhai16hadtheCC genotype.Among 60 extreme materials of the G* hirsutum-G. barbadense backcross populations，there was a significant difference in fiber length between the CC genotype（37 materials） and the TT genotype （23 materials）（ P<0.05 ），and the average fiber length of materials withtheTTgenotypewasshorterthanthatofmaterialswiththeCC genotype.Furthermore，asimilarpaternwasobserved in305 G. hirsutummaterialsand145G.barbadensematerials：theaveragefiberlengthofmaterialswiththeTTgenotypewasstillsorter than that of materials with the CC genotype （ P<0.05 ）. In conclusion，the GhCRK26-I931 SNP molecular marker can be effectivelyusedintheselectivebredingforcottonfiberlength，andtheCC genotypehasapositiveeffectonimprovingfiberength. This studynotonlyopens upanew avenueforthe breding of high-qualitycottonvarietiesbut alsoprovides areliable tol for marker-assisted selection of cotton fiber quality.

Key words： cotton;fiber length；CRK26 gene；KASP-SNP molecular marker

棉花作为全球重要的经济作物，不仅是关系国计民生的重要战略物资，在世界经济发展中也占据举足轻重的地位，其纤维品质的优劣直接决定了纺织工业的生产效率和产品附加值[1-3]。（剩余13783字）