EK2 稀释液对临武鸭精子活力及人工授精效果的影响

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中图分类号：S834

文献标识码：A

文章编号：1006-060X（2026）03-0099-04

Effect of EK2 Diluent on Sperm Motility and Artificial Insemination Outcomes in Linwu Ducks

LIU Bo-cheng 1 ， ZHU Li-jun 1 ， YAN Hai-feng 1 ， SHI Wu-ping 2 ， JIANG Gui-tao 1 ， GAO Chu-ze 1 ， WAN Wei-can 1 ， HE Xu 1 ， ZHANG Xian-xin 1

Hunan Institute of Animal Husbandry and Veterinary Science， Changsha 410131， PRC; 2. Songbai Town Agricultural Comprehensive Service Center， Yongshun 416708， PRC）

Abstract： This study aimed to explore the effect of different dilution ratios of duck semen on artificial insemination. Semen was collected from Linwu ducks and diluted at ratios of 1 ： 1 and 1 ： 3 with EK2 diluent for artificial insemination. Post-dilution sperm motility， sperm viability， and sperm motility parameters [straight-line velocity （VSL）， curvilinear velocity （VCL）， and average path velocity （VAP）]， fertility rate， and hatchability were assessed. The results showed that at the time point of 4 h post-dilution， the sperm motility， viability， and motility performance of both 1 ： 1 and 1 ： 3 dilution groups were extremely significantly higher than that in the normal saline dilution group. At the time point of 6 h post-dilution， sperm motility in the 1 ： 3 dilution group was extremely significantly higher than that in the 1 ： 1 dilution group. During 1–6 d post-insemination， the average fertility rates and hatchability of the 1 ： 1 dilution， 1 ： 3 dilution， and original semen groups had no significant differences. During 7–12 d， the average fertility rate of the original semen group was extremely significantly higher than those of the 1 ： 1 and 1 ： 3 dilution groups. Daily fertility rate analysis revealed that the 1 ： 1 and 1 ： 3 dilution groups had higher fertility rate than the original semen group during 1–4 d and had lower fertility rate during 5–12 d. In conclusion， ideal fertility can be achieved by inseminating semen diluted with EK2 diluent at a ratio of 1 ： 3 or 1 ： 1 （with an effective sperm count of no less than 1.0×108 ）. Considering the fertility trend， it was recommended to perform artificial insemination every 4 d in production.

Key words： Linwu ducks; EK2 diluent; artificial insemination; fertility rate

人工授精技术能够显著提高种禽利用率、降低疾病传播风险并提升养殖效益，在现代家禽育种与规模化生产中发挥着关键作用 [1] 。（剩余6286字）