基于 ISSR 分子标记的 16 份桑树种质资源遗传多样性分析

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中图分类号：S888.3

文献标识码：A

文章编号：1006-060X（2026）03-0094-05

Genetic Diversity Analysis of 16 Mulberry （Morus L.） Germplasm Accessions Based on ISSR Markers

SHEN Lin-jing， JIANG Yong-bing， JIANG Shi-meng， CAO Hui， ZOU Xiang-yue， DUAN Jun-yi， LIU Fei-chi， WANG Ming

（Hunan Institute of Cotton and Sericulture， Changsha 410127， PRC）

Abstract： This study employed inter-simple sequence repeat （ISSR） markers to investigate the genetic diversity of 16 mulberry germplasm accessions from the mulberry germplasm nursery of Hunan Institute of Cotton and Sericulture. Seven effective ISSR primers were screened out， and amplification with these primers yielded a total of 73 bands， among which 60 were polymorphic bands， with the polymorphic loci accounting for 82.19%. The observed number of alleles， effective number of alleles， Nei's gene diversity index， and Shannon's information index were 1.821 9， 1.479 2， 0.280 0， and 0.420 5， respectively， which indicated rich genetic diversity of the mulberry germplasm accessions. The 16 mulberry germplasm accessions showed the genetic distances ranging from 0.042 0 to 0.764 1 and the genetic similarity coefficients ranging from 0.465 8 to 0.958 9. 'Yu 71-1 6' and 'Yu 71-1 7' showed the shortest genetic distance （0.042 0）， with the closest genetic relationship. 'Yu 71-1 4' and 'Xiangsang 6' exhibited the longest genetic distance （0.764 1）， which indicated the farthest genetic relationship. The results of UPGMA cluster analysis indicated that the 16 mulberry germplasm accessions could be assigned into five major groups at the genetic distance of 0.71.

Key words： mulberry; germplasm accession; genetic diversity; ISSR marker

桑树（Morus alba L.）属于桑科桑属，是蚕桑产业的重要作物，也是兼具生态、经济与药用多重价值的多年生木本植物。（剩余7270字）