油酸诱导粗毛纤孔菌总三萜物质合成与防御酶相关性研究

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中图分类号：S567.3 文献标志码：A

Correlation between triterpenoid synthesis and defense enzyme activities in Inonotus hispidus induced by oleic acid

ZHANG Xinyue1’² ， SUN Xiaofan1，²，HU Yixin1²， ZHANG Tianyang² ，

LI Dehai1'² ， YU Yuyang² ， ZHOU Lin²

（1. State Key Laboratoryof Woody Oil Resources Utilization，Northeast Forestry University，Harbinl50040，China;2. College of Life Sciences，Northeast Forestry University，Harbin 15o04O，China）

ABSTRACT： Inonotus hispidus is a medicinal fungus whose main pharmacologicall active components are triterpenoids， which posess various biologicalactivities such asantioxidantandantitumor efects.However，under natural conditions， itsfruiting bodies exhibitalong growth cycle and lowtriterpenoidcontent，which limits itsmedicinal development potential.Toexplore methods forincreasing the yieldof triterpenoids in Inonotus hispidusandtheirregulatory mechanisms， this studyused oleic acid asan inducer，examined the activitychangesof key defense enzymes inthe mycelia，and analyzed their dynamic relationship with triterpenoid biosynthesis. The results showed that 0.4% oleic acid under liquid culture conditions significantly increased the total triterpenoid content，with the triterpenoid synthesis rate peaking at 84h and the total triterpenoid content reaching its maximum at 144h ， Oleic acid activated the activities of intracellular defense enzymes，including superoxide dismutase （SOD），catalase（CAT），and peroxidase （POD）.During the early stage of the stressresponse，increased SODactivities were strongly positivelycorrelated withthe triterpenoid synthesisrate.Asthe duction time was prolonged，the activities of thethreedefenseenzymesandthetriterpenoid synthesisrateshowedasignificantpositive corelation.These findings providea theoretical foundation for enhancing the biosynthesisof triterpenoid natural products.

KEY WORDs： Inonotus his pidus ; oleic acid; triterpenoids;defense enzyme

粗毛纤孔菌（Inonotushispidus）又名粗毛黄褐孔菌，为多孔菌目多孔菌科纤毛孔属真菌[1]，多寄生于阔叶树木，如桑树、水曲柳等。（剩余16138字）